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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunoprecipitation [1]
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Validation data
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- Product number
- TA347075 - Provider product page
- Provider
- OriGene
- Product name
- Mouse Monoclonal ZAP-70 Antibody
- Antibody type
- Monoclonal
- Description
- Mouse Monoclonal ZAP-70 Antibody
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- ZAP70
- Isotype
- IgG
- Antibody clone number
- 4B2-C1-H3
- Vial size
- 100 µl
- Concentration
- NULL
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot detection of ZAP-70 in CEM and Jurkat cell lysates,negative in the Ramos cell lysates using ZAP-70 mouse mAb (1:1000 diluted).Predicted band size:70KDa.Observed band size:70KDa.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunoprecipitation analysis of Jurkat cell lysates (ZAP-70 positive expression cell line)and Ramos cell lysates(ZAP-70 negative expression cell line) using ZAP-70 mouse mAb.
- Validation comment
- IP
