Antibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Flow cytometry [1]
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Validation data
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- Product number
- MHZAP7004 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Zap-70 Monoclonal Antibody (1E7.2), PE
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- R-phycoerythrin (PE) is a stable and highly soluble phycobiliprotein which provides maximal absorbance and fluorescence without susceptibility to internal or external fluorescence quenching, thus providing an exceptional quantum yields and molar extinction coefficients.
- Reactivity
- Human, Mouse
- Host
- Mouse
- Conjugate
- Yellow dye
- Isotype
- IgG
- Antibody clone number
- 1E7.2
- Vial size
- 500 µL
- Storage
- 4° C, store in dark
Submitted references CMV Primes Functional Alternative Signaling in Adaptive Δg NK Cells but Is Subverted by Lentivirus Infection in Rhesus Macaques.
Pitfalls and limitations of ZAP-70 detection in chronic lymphocytic leukemia.
Methodological comparison of two anti-ZAP-70 antibodies.
Shah SV, Manickam C, Ram DR, Kroll K, Itell H, Permar SR, Barouch DH, Klatt NR, Reeves RK
Cell reports 2018 Dec 4;25(10):2766-2774.e3
Cell reports 2018 Dec 4;25(10):2766-2774.e3
Pitfalls and limitations of ZAP-70 detection in chronic lymphocytic leukemia.
Vroblova V, Smolej L, Krejsek J
Hematology (Amsterdam, Netherlands) 2012 Sep;17(5):268-74
Hematology (Amsterdam, Netherlands) 2012 Sep;17(5):268-74
Methodological comparison of two anti-ZAP-70 antibodies.
Degheidy HA, Venzon DJ, Farooqui MZ, Abbasi F, Arthur DC, Wilson WH, Wiestner A, Stetler-Stevenson MA, Marti GE
Cytometry. Part B, Clinical cytometry 2011 Sep;80(5):300-8
Cytometry. Part B, Clinical cytometry 2011 Sep;80(5):300-8
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Human peripheral blood from normal donors was stained using a combination of anti-human CD3 monoclonal antibody (clone: S4.1), for the cell surface antigens, and anti-human/mouse Zap-70 monoclonal antibody (clone: 1E7.2), for intracellular detection. The cells were fixed and/or permeabilized using Fix and Perm solution (Product # GAS-003). The profiles represent lymphocyte gate.
- Conjugate
- Yellow dye