MHZAP7004-4

antibody from Invitrogen Antibodies

Targeting: ZAP70 SRK, STD, ZAP-70

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

MHZAP7004-4

Provider

Invitrogen Antibodies

Product name

Zap-70 Monoclonal Antibody (1E7.2), PE

Antibody type

Monoclonal

Antigen

Other

Description

R-phycoerythrin (PE) is a stable and highly soluble phycobiliprotein which provides maximal absorbance and fluorescence without susceptibility to internal or external fluorescence quenching, thus providing an exceptional quantum yields and molar extinction coefficients.

Reactivity

Human, Mouse

Host

Mouse

Conjugate

Yellow dye

Isotype

IgG

Antibody clone number

1E7.2

Vial size

2 mL

Storage

4° C, store in dark

References

Submitted references

CMV Primes Functional Alternative Signaling in Adaptive Δg NK Cells but Is Subverted by Lentivirus Infection in Rhesus Macaques.

Shah SV, Manickam C, Ram DR, Kroll K, Itell H, Permar SR, Barouch DH, Klatt NR, Reeves RK
Cell reports 2018 Dec 4;25(10):2766-2774.e3

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Pitfalls and limitations of ZAP-70 detection in chronic lymphocytic leukemia.

Vroblova V, Smolej L, Krejsek J
Hematology (Amsterdam, Netherlands) 2012 Sep;17(5):268-74

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Methodological comparison of two anti-ZAP-70 antibodies.

Degheidy HA, Venzon DJ, Farooqui MZ, Abbasi F, Arthur DC, Wilson WH, Wiestner A, Stetler-Stevenson MA, Marti GE
Cytometry. Part B, Clinical cytometry 2011 Sep;80(5):300-8

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Human peripheral blood from normal donors was stained using a combination of anti-human CD3 monoclonal antibody (clone: S4.1), for the cell surface antigens, and anti-human/mouse Zap-70 monoclonal antibody (clone: 1E7.2), for intracellular detection. The cells were fixed and/or permeabilized using Fix and Perm solution (Product # GAS-003). The profiles represent lymphocyte gate.

Conjugate

Yellow dye