Antibody data
- Antibody Data
- Antigen structure
- References [13]
- Comments [0]
- Validations
- Flow cytometry [1]
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- Product number
- MHZAP7020 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Zap-70 Monoclonal Antibody (1E7.2), Alexa Fluor™ 488
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Based on our testing, publications, and results reported from customers, the Alexa Fluor® 488 dye provides the best fluorescein (FITC) substitute.
- Reactivity
- Human, Mouse
- Host
- Mouse
- Conjugate
- Green dye
- Isotype
- IgG
- Antibody clone number
- 1E7.2
- Vial size
- 500 µL
- Storage
- 4° C, store in dark
Submitted references Engineering a Dual Small Molecule Gated ZAP70 Switch in T Cells.
A novel human autoimmune syndrome caused by combined hypomorphic and activating mutations in ZAP-70.
Combined normal donor and CLL: Single tube ZAP-70 analysis.
Tipping the Noxa/Mcl-1 balance overcomes ABT-737 resistance in chronic lymphocytic leukemia.
Quantitative analysis of protein phosphorylations and interactions by multi-colour IP-FCM as an input for kinetic modelling of signalling networks.
Sorafenib induces cell death in chronic lymphocytic leukemia by translational downregulation of Mcl-1.
Cluster analysis of immunophenotypic data: the example of chronic lymphocytic leukemia.
Prognostic impact of ZAP-70 expression in chronic lymphocytic leukemia: mean fluorescence intensity T/B ratio versus percentage of positive cells.
High lymphoid enhancer-binding factor-1 expression is associated with disease progression and poor prognosis in chronic lymphocytic leukemia.
ZAP-70 expression, as detected by immunohistochemistry on bone marrow biopsies from early-phase CLL patients, is a strong adverse prognostic factor.
A scoring system based on the expression of six surface molecules allows the identification of three prognostic risk groups in B-cell chronic lymphocytic leukemia.
ZAP-70 by flow cytometry: a comparison of different antibodies, anticoagulants, and methods of analysis.
Clinical significance of ZAP-70 protein expression in B-cell chronic lymphocytic leukemia.
Wong NML, Wong WW
ACS synthetic biology 2018 Apr 20;7(4):969-977
ACS synthetic biology 2018 Apr 20;7(4):969-977
A novel human autoimmune syndrome caused by combined hypomorphic and activating mutations in ZAP-70.
Chan AY, Punwani D, Kadlecek TA, Cowan MJ, Olson JL, Mathes EF, Sunderam U, Fu SM, Srinivasan R, Kuriyan J, Brenner SE, Weiss A, Puck JM
The Journal of experimental medicine 2016 Feb 8;213(2):155-65
The Journal of experimental medicine 2016 Feb 8;213(2):155-65
Combined normal donor and CLL: Single tube ZAP-70 analysis.
Degheidy HA, Venzon DJ, Farooqui MZ, Abbasi F, Arthur DC, Wilson WH, Wiestner A, Stetler-Stevenson MA, Marti GE
Cytometry. Part B, Clinical cytometry 2012 Mar;82(2):67-77
Cytometry. Part B, Clinical cytometry 2012 Mar;82(2):67-77
Tipping the Noxa/Mcl-1 balance overcomes ABT-737 resistance in chronic lymphocytic leukemia.
Tromp JM, Geest CR, Breij EC, Elias JA, van Laar J, Luijks DM, Kater AP, Beaumont T, van Oers MH, Eldering E
Clinical cancer research : an official journal of the American Association for Cancer Research 2012 Jan 15;18(2):487-98
Clinical cancer research : an official journal of the American Association for Cancer Research 2012 Jan 15;18(2):487-98
Quantitative analysis of protein phosphorylations and interactions by multi-colour IP-FCM as an input for kinetic modelling of signalling networks.
Deswal S, Schulze AK, Höfer T, Schamel WW
PloS one 2011;6(7):e22928
PloS one 2011;6(7):e22928
Sorafenib induces cell death in chronic lymphocytic leukemia by translational downregulation of Mcl-1.
Huber S, Oelsner M, Decker T, zum Büschenfelde CM, Wagner M, Lutzny G, Kuhnt T, Schmidt B, Oostendorp RA, Peschel C, Ringshausen I
Leukemia 2011 May;25(5):838-47
Leukemia 2011 May;25(5):838-47
Cluster analysis of immunophenotypic data: the example of chronic lymphocytic leukemia.
Zucchetto A, Cattarossi I, Nanni P, Zaina E, Prato G, Gilestro M, Marconi D, Bulian P, Rossi FM, Del Vecchio L, Omedè P, Geuna M, Del Poeta G, Gattei V
Immunology letters 2011 Jan 30;134(2):137-44
Immunology letters 2011 Jan 30;134(2):137-44
Prognostic impact of ZAP-70 expression in chronic lymphocytic leukemia: mean fluorescence intensity T/B ratio versus percentage of positive cells.
Rossi FM, Del Principe MI, Rossi D, Irno Consalvo M, Luciano F, Zucchetto A, Bulian P, Bomben R, Dal Bo M, Fangazio M, Benedetti D, Degan M, Gaidano G, Del Poeta G, Gattei V
Journal of translational medicine 2010 Mar 8;8:23
Journal of translational medicine 2010 Mar 8;8:23
High lymphoid enhancer-binding factor-1 expression is associated with disease progression and poor prognosis in chronic lymphocytic leukemia.
Erdfelder F, Hertweck M, Filipovich A, Uhrmacher S, Kreuzer KA
Hematology reports 2010 Jan 26;2(1):e3
Hematology reports 2010 Jan 26;2(1):e3
ZAP-70 expression, as detected by immunohistochemistry on bone marrow biopsies from early-phase CLL patients, is a strong adverse prognostic factor.
Zanotti R, Ambrosetti A, Lestani M, Ghia P, Pattaro C, Remo A, Zanetti F, Stella S, Perbellini O, Prato G, Guida G, Caligaris-Cappio F, Menestrina F, Pizzolo G, Chilosi M
Leukemia 2007 Jan;21(1):102-9
Leukemia 2007 Jan;21(1):102-9
A scoring system based on the expression of six surface molecules allows the identification of three prognostic risk groups in B-cell chronic lymphocytic leukemia.
Zucchetto A, Bomben R, Dal Bo M, Sonego P, Nanni P, Rupolo M, Bulian P, Dal Maso L, Del Poeta G, Del Principe MI, Degan M, Gattei V
Journal of cellular physiology 2006 May;207(2):354-63
Journal of cellular physiology 2006 May;207(2):354-63
ZAP-70 by flow cytometry: a comparison of different antibodies, anticoagulants, and methods of analysis.
Best OG, Ibbotson RE, Parker AE, Davis ZA, Orchard JA, Oscier DG
Cytometry. Part B, Clinical cytometry 2006 Jul 15;70(4):235-41
Cytometry. Part B, Clinical cytometry 2006 Jul 15;70(4):235-41
Clinical significance of ZAP-70 protein expression in B-cell chronic lymphocytic leukemia.
Del Principe MI, Del Poeta G, Buccisano F, Maurillo L, Venditti A, Zucchetto A, Marini R, Niscola P, Consalvo MA, Mazzone C, Ottaviani L, Panetta P, Bruno A, Bomben R, Suppo G, Degan M, Gattei V, de Fabritiis P, Cantonetti M, Lo Coco F, Del Principe D, Amadori S
Blood 2006 Aug 1;108(3):853-61
Blood 2006 Aug 1;108(3):853-61
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Human peripheral blood from normal donors was stained using a combination of anti-human CD3 monoclonal antibody (clone: S4.1), for the cell surface antigens, and anti-human/mouse Zap-70 monoclonal antibody (clone: 1E7.2), for intracellular detection. The cells were fixed and/or permeabilized using Fix and Perm solution (Product # GAS-003). The profiles represent lymphocyte gate.
- Conjugate
- Green dye