MHZAP7020

antibody from Invitrogen Antibodies

Targeting: ZAP70 SRK, STD, ZAP-70

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

MHZAP7020

Provider

Invitrogen Antibodies

Product name

Zap-70 Monoclonal Antibody (1E7.2), Alexa Fluor™ 488

Antibody type

Monoclonal

Antigen

Other

Description

Based on our testing, publications, and results reported from customers, the Alexa Fluor® 488 dye provides the best fluorescein (FITC) substitute.

Reactivity

Human, Mouse

Host

Mouse

Conjugate

Green dye

Isotype

IgG

Antibody clone number

1E7.2

Vial size

500 µL

Storage

4° C, store in dark

References

Submitted references

Engineering a Dual Small Molecule Gated ZAP70 Switch in T Cells.

Wong NML, Wong WW
ACS synthetic biology 2018 Apr 20;7(4):969-977

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A novel human autoimmune syndrome caused by combined hypomorphic and activating mutations in ZAP-70.

Chan AY, Punwani D, Kadlecek TA, Cowan MJ, Olson JL, Mathes EF, Sunderam U, Fu SM, Srinivasan R, Kuriyan J, Brenner SE, Weiss A, Puck JM
The Journal of experimental medicine 2016 Feb 8;213(2):155-65

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Combined normal donor and CLL: Single tube ZAP-70 analysis.

Degheidy HA, Venzon DJ, Farooqui MZ, Abbasi F, Arthur DC, Wilson WH, Wiestner A, Stetler-Stevenson MA, Marti GE
Cytometry. Part B, Clinical cytometry 2012 Mar;82(2):67-77

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Tipping the Noxa/Mcl-1 balance overcomes ABT-737 resistance in chronic lymphocytic leukemia.

Tromp JM, Geest CR, Breij EC, Elias JA, van Laar J, Luijks DM, Kater AP, Beaumont T, van Oers MH, Eldering E
Clinical cancer research : an official journal of the American Association for Cancer Research 2012 Jan 15;18(2):487-98

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Quantitative analysis of protein phosphorylations and interactions by multi-colour IP-FCM as an input for kinetic modelling of signalling networks.

Deswal S, Schulze AK, Höfer T, Schamel WW
PloS one 2011;6(7):e22928

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Sorafenib induces cell death in chronic lymphocytic leukemia by translational downregulation of Mcl-1.

Huber S, Oelsner M, Decker T, zum Büschenfelde CM, Wagner M, Lutzny G, Kuhnt T, Schmidt B, Oostendorp RA, Peschel C, Ringshausen I
Leukemia 2011 May;25(5):838-47

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Cluster analysis of immunophenotypic data: the example of chronic lymphocytic leukemia.

Zucchetto A, Cattarossi I, Nanni P, Zaina E, Prato G, Gilestro M, Marconi D, Bulian P, Rossi FM, Del Vecchio L, Omedè P, Geuna M, Del Poeta G, Gattei V
Immunology letters 2011 Jan 30;134(2):137-44

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Prognostic impact of ZAP-70 expression in chronic lymphocytic leukemia: mean fluorescence intensity T/B ratio versus percentage of positive cells.

Rossi FM, Del Principe MI, Rossi D, Irno Consalvo M, Luciano F, Zucchetto A, Bulian P, Bomben R, Dal Bo M, Fangazio M, Benedetti D, Degan M, Gaidano G, Del Poeta G, Gattei V
Journal of translational medicine 2010 Mar 8;8:23

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High lymphoid enhancer-binding factor-1 expression is associated with disease progression and poor prognosis in chronic lymphocytic leukemia.

Erdfelder F, Hertweck M, Filipovich A, Uhrmacher S, Kreuzer KA
Hematology reports 2010 Jan 26;2(1):e3

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ZAP-70 expression, as detected by immunohistochemistry on bone marrow biopsies from early-phase CLL patients, is a strong adverse prognostic factor.

Zanotti R, Ambrosetti A, Lestani M, Ghia P, Pattaro C, Remo A, Zanetti F, Stella S, Perbellini O, Prato G, Guida G, Caligaris-Cappio F, Menestrina F, Pizzolo G, Chilosi M
Leukemia 2007 Jan;21(1):102-9

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A scoring system based on the expression of six surface molecules allows the identification of three prognostic risk groups in B-cell chronic lymphocytic leukemia.

Zucchetto A, Bomben R, Dal Bo M, Sonego P, Nanni P, Rupolo M, Bulian P, Dal Maso L, Del Poeta G, Del Principe MI, Degan M, Gattei V
Journal of cellular physiology 2006 May;207(2):354-63

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ZAP-70 by flow cytometry: a comparison of different antibodies, anticoagulants, and methods of analysis.

Best OG, Ibbotson RE, Parker AE, Davis ZA, Orchard JA, Oscier DG
Cytometry. Part B, Clinical cytometry 2006 Jul 15;70(4):235-41

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Clinical significance of ZAP-70 protein expression in B-cell chronic lymphocytic leukemia.

Del Principe MI, Del Poeta G, Buccisano F, Maurillo L, Venditti A, Zucchetto A, Marini R, Niscola P, Consalvo MA, Mazzone C, Ottaviani L, Panetta P, Bruno A, Bomben R, Suppo G, Degan M, Gattei V, de Fabritiis P, Cantonetti M, Lo Coco F, Del Principe D, Amadori S
Blood 2006 Aug 1;108(3):853-61

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Human peripheral blood from normal donors was stained using a combination of anti-human CD3 monoclonal antibody (clone: S4.1), for the cell surface antigens, and anti-human/mouse Zap-70 monoclonal antibody (clone: 1E7.2), for intracellular detection. The cells were fixed and/or permeabilized using Fix and Perm solution (Product # GAS-003). The profiles represent lymphocyte gate.

Conjugate

Green dye