Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- PB9968 - Provider product page
- Provider
- Boster Biological Technology
- Product name
- Anti-ZAP70 Antibody Picoband™
- Antibody type
- Polyclonal
- Description
- Polyclonal antibody for ZAP70 detection. Host: Rabbit.Size: 100μg/vial. Tested applications: IHC-P. Reactive species: Human. ZAP70 information: Molecular Weight: 69872 MW; Subcellular Localization: Cytoplasm . Cell membrane ; Peripheral membrane protein . In quiescent T-lymphocytes, it is cytoplasmic. Upon TCR activation, it is recruited at the plasma membrane by interacting with CD247/CD3Z. Colocalizes together with RHOH in the immunological synapse. RHOH is required for its proper localization to the cell membrane and cytoskeleton fractions in the thymocytes (By similarity); Tissue Specificity: Expressed in T- and natural killer cells. Also present in early thymocytes and pro/pre B-cells.
- Reactivity
- Human
- Host
- Rabbit
- Vial size
- 100μg/vial
- Concentration
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
- Storage
- At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquoted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
- Handling
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
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Supportive validation
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- Boster Biological Technology (provider)
- Main image
- Experimental details
- Western blot analysis of ZAP70 expression in JURKAT whole cell lysates (lane 1) and CEM whole cell lysates (lane 2). ZAP70 at 70KD was detected using rabbit anti- ZAP70 Antigen Affinity purified polyclonal antibody (Catalog # PB9968) at 0.5 μg/mL. The blot was developed using chemiluminescence (ECL) method (Catalog # EK1002).
- Additional image