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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Western blot [2]
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- Product number
- MAB3664 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#MAB3664, RRID:AB_10677588
- Product name
- ZAP70 monoclonal antibody, clone ZAP-03
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against partial recombinant ZAP70.
- Isotype
- IgG
- Antibody clone number
- ZAP-03
- Storage
- Store at 4°C. Do not freeze.Aliquot to avoid repeated freezing and thawing.
Submitted references Detection of HLA-G by a specific sandwich ELISA using monoclonal antibodies G233 and 56B.
HLA Class I protein expression in the human placenta.
A human histocompatibility leukocyte antigen (HLA)-G-specific receptor expressed on all natural killer cells.
Van Lierop MJ, Wijnands F, Loke YW, Emmer PM, Lukassen HG, Braat DD, van der Meer A, Mosselman S, Joosten I
Molecular human reproduction 2002 Aug;8(8):776-84
Molecular human reproduction 2002 Aug;8(8):776-84
HLA Class I protein expression in the human placenta.
Blaschitz A, Hutter H, Dohr G
Early pregnancy (Online) 2001 Jan;5(1):67-9
Early pregnancy (Online) 2001 Jan;5(1):67-9
A human histocompatibility leukocyte antigen (HLA)-G-specific receptor expressed on all natural killer cells.
Rajagopalan S, Long EO
The Journal of experimental medicine 1999 Apr 5;189(7):1093-100
The Journal of experimental medicine 1999 Apr 5;189(7):1093-100
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western Blotting analysis (reducing conditions) of HPB-ALL (human peripheral blood T cell leukemia cell line).Lane 1, 2, 3, 4 : Immunostaining with dilution range of ZAP70 monoclonal antibody, clone ZAP-03 (Cat # MAB3664) ; 4 ug/mL (1), 2 ug/mL (2), 1 ug/mL (3), 0.5 ug/mL (4).Lane 5 : Immunostaining with anti-ZAP70 comparative antibody.Lane 6 : Immunostaining with Isotype mouse IgG1 control.
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western Blotting analysis (reducing conditions) of HPB-ALL (human peripheral blood T cell leukemia cell line) (1, 3) and RAMOS (human Burkitt lymphoma cell line) (2, 4).Lane 1, 2 : Immunostaining with ZAP70 monoclonal antibody, clone ZAP-03 (Cat # MAB3664 ; 0.5 ug/mL).Lane 3, 4 : Immunostaining with Isotype mouse IgG1 control.
