12-6695-42

antibody from Invitrogen Antibodies
Targeting: ZAP70 SRK, STD, ZAP-70
Provider product page for 12-6695-42

Antibody data

Product number
12-6695-42 - Provider product page
Provider
Invitrogen Antibodies
Product name
Zap-70 Monoclonal Antibody (1E7.2), PE, eBioscience™
Antibody type
Monoclonal
Antigen
Other
Description
Description: The 1E7.2 antibody reacts with human and mouse ZAP-70, the TCR?-associated protein-70. ZAP-70 is a cytosolic protein tyrosine kinase (PTK) and a member of the Syk family of proteins. It is expressed in T and NK cells and is required for TCR signaling and development. ZAP-70 interacts with the TCR complex by binding to tyrosine-phosphorylated immunoreceptor tyrosine-based activation motifs (ITAMs) present in the invariant subunits of the TCR complex. Following activation, ZAP-70 is phosphorylated on several tyrosine residues by two mechanisms; an autophosphorylation and a transphosphorylation by the Src family tyrosine kinase Lck1-3. Tyrosine phosphorylation of ZAP-70 correlates to its increased kinase activity and triggers downstream signaling events. Mutations in ZAP-70 have been shown to result in a form of Severe Combined Immunodeficiency Syndrome (SCID) in humans. 1E7.2 was generated against a KLH-peptide sequence corresponding to the human ZAP-70 amino acid residues 282-307. While ZAP-70 is normally expressed in T and NK cells, several recent studies have also shown high correlation of ZAP-70 positive expression with mutated IgVH expression in B-chronic lymphocytic leukemia (CCL). In conclusion, the expression of ZAP-70, which can be measured by intracellular flow cytometry, may serve as a prognostic marker for B-CLL.
Conjugate
Yellow dye
Antibody clone number
1E7.2
Concentration
5 µL/Test
ZAP70 protein structure - 12-6695-42 shown in red.