M01857-3

antibody from Boster Biological Technology

Targeting: ZAP70 SRK, STD, ZAP-70

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Flow cytometry (Data presented on provider website)

Antibody Data

Product number

M01857-3

Provider

Boster Biological Technology

Product name

Anti-Beta Tubulin Antibody Picoband™(monoclonal, 5E4)

Antibody type

Monoclonal

Description

Mouse IgG monoclonal antibody for Beta Tubulin detection. Tested with WB, ICC/IF, FCM in Human;Mouse;Rat.

Reactivity

Human, Mouse, Rat

Host

Mouse

Isotype

IgG

Antibody clone number

5.00E+04

Vial size

100μg/vial

Storage

At -20ËšC for one year. After reconstitution, at 4ËšC for one month. It can also be aliquoted and stored frozen at -20ËšC for a longer time. Avoid repeated freezing and thawing.

Handling

Add 0.2ml of distilled water will yield a concentration of 500μg/ml.

References

Submitted references

Enhancing osteoblast proliferation and bone regeneration by poly (amino acid)/selenium-doped hydroxyapatite.

Wei X, Zhang Z, Wang L, Yan L, Yan Y, Wang C, Peng H, Fan X
Biomedical materials (Bristol, England) 2024 Apr 4;19(3)

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Identification of Critical Amino Acids of Coxsackievirus A10 Associated with Cell Tropism and Viral RNA Release during Uncoating.

Pei J, Liu RL, Yang ZH, Du YX, Qian SS, Meng SL, Guo J, Zhang B, Shen S
Viruses 2023 Oct 18;15(10)

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HMGB1 in the mPFC governs comorbid anxiety in neuropathic pain.

Du Y, Xu CL, Yu J, Liu K, Lin SD, Hu TT, Qu FH, Guo F, Lou GD, Nishibori M, Hu WW, Chen Z, Zhang SH
The journal of headache and pain 2022 Aug 16;23(1):102

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Ibrutinib targets microRNA-21 in multiple myeloma cells by inhibiting NF-κB and STAT3.

Ma J, Gong W, Liu S, Li Q, Guo M, Wang J, Wang S, Chen N, Wang Y, Liu Q, Zhao H
Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine 2018 Jan;40(1):1010428317731369

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NEAT1 upregulates EGCG-induced CTR1 to enhance cisplatin sensitivity in lung cancer cells.

Jiang P, Wu X, Wang X, Huang W, Feng Q
Oncotarget 2016 Jul 12;7(28):43337-43351

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Ezetimibe-mediated protection of vascular smooth muscle cells from cholesterol accumulation through the regulation of lipid metabolism-related gene expression.

Gong YZ, Sun SW, Yuan HY, Xie XJ, Tuo QH, Yang XF, Tan X, Zheng XL, Xiong Y, Liao DF
Pharmacology 2014;94(5-6):214-22

Western blot

Supportive validation

Submitted by

Boster Biological Technology (provider)

Main image

Experimental details

Western blot analysis of Beta Tubulin using anti-Beta Tubulin antibody (M01857-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HEK293 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human HEPG2 whole cell lysates, Lane 4: human HL-60 whole cell lysates, Lane 5: human Raji whole cell lysates, Lane 6: rat brain tissue lysates, Lane 7: mouse brain tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-Beta Tubulin antigen affinity purified monoclonal antibody (Catalog # M01857-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for Beta Tubulin at approximately 55KD. The expected band size for Beta Tubulin is at 55KD.

Additional image