22207-1-AP

antibody from Invitrogen Antibodies

Targeting: ZAP70 SRK, STD, ZAP-70

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

22207-1-AP

Provider

Invitrogen Antibodies

Product name

ZAP70 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: LVEYLKLKA DGLIYCLKEA CPNSSASNAS GAAAPTLPAH PSTLTHPQRR IDTLNSDGYT PEPARITSPD KPRPMPMDTS VYESPYSDPE ELKDKKLFLK RDNLLIADIE LGCGNFGSVR QGVYRMRKKQ IDVAIKVLKQ GTEKADTEEM MREAQIMHQL (109-267 aa encoded by BC039039)

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.39 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Jurkat cells were subjected to SDS PAGE followed by western blot with 22207-1-AP ( ZAP70 Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

RAW264.7 cells were subjected to SDS PAGE followed by western blot with 22207-1-AP (ZAP70 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of RAW 264.7 cells using 22207-1-AP (ZAP70 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human lymphoma using 22207-1-AP (ZAP70 antibody) at dilution of 1:50 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human lymphoma using 22207-1-AP (ZAP70 antibody) at dilution of 1:50 (under 40x lens).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-ZAP70 (IP:22207-1-AP, 4ug; Detection:22207-1-AP 1:1000) with Jurkat cells lysate 2800ug.