MA5-12624

antibody from Invitrogen Antibodies

Targeting: ZAP70 SRK, STD, ZAP-70

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-12624

Provider

Invitrogen Antibodies

Product name

Zap-70 Monoclonal Antibody (2F3.2)

Antibody type

Monoclonal

Antigen

Other

Description

MA5-12624 targets ZAP-70 in IHC (P) and WB applications and shows reactivity with Human samples. The MA5-12624 immunogen is gST-fusion to tandem SH2 domains of human ZAP-70 corresponding to residues 1-254.

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

2F3.2

Vial size

500 µL

Concentration

0.2 mg/mL

Storage

4° C

References

Submitted references

Comparison of the mRNA expression profile of B-cell receptor components in normal CD5-high B-lymphocytes and chronic lymphocytic leukemia: a key role of ZAP70.

Gladkikh AA, Potashnikova DM, Tatarskiy V Jr, Yastrebova M, Khamidullina A, Barteneva N, Vorobjev I
Cancer medicine 2017 Dec;6(12):2984-2997

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot of ZAP-70 using ZAP-70 Monoclonal Antibody (Product # MA5-12624) on Jurkat Cells.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis was performed on membrane enriched extracts (30 µg lysate) of Jurkat (Lane 1), MOLT-4 (Lane 2) and Ramos (Lane 3). The blot was probed with ZAP70 Mouse Monoclonal Antibody (Product # MA5-12624, 1 µg/mL) and detected by chemiluminescence using Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A28177, 0.25 µg/mL, 1:4000 dilution). A 69 kDa band corresponding to ZAP70 was observed in Jurkat and MOLT-4 cell lines. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 10 % Bis-Tris gel (Product # NP0321BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard  (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® 2 Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary Antibody following blocking with 5 % skimmed milk. Chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate (Product # 32106).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Formalin-Fixed, paraffin-embedded human tonsil stained with anti-Zap-70 using peroxidase-conjugate and AEC chromogen. Note staining of T-Cells.