Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Immunoprecipitation [2]
- Flow cytometry [1]
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Validation data
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- Product number
- NBP1-18896 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-18896, RRID:AB_1625969
- Product name
- Rabbit Polyclonal ZAP70 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C. Do not freeze.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: ZAP70 [p Tyr319] Antibody [NBP1-18896] - Whole cell lysate from Jurkat cells that had been treated with hydrogen peroxide. ZAP70 antibodies NBP1-18896, NBP1-1889, and NBP1-18898 used for IP at 3 mcg/mg lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: ZAP70 [p Tyr319] Antibody [NBP1-18896] - Jurkat WC Lysate treated with hydrogen peroxide (+) or mock treated (-). NB100-97826 was used to IP ZAP70. The IPs were either treated with phosphatase (+) or mock (-) treated.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: ZAP70 [p Tyr319] Antibody [NBP1-18896] - Flow cytometric analysis of phospho-Zap70 (Y319). Jurkat cells were treated with H2O2 for 3 minutes. Cells were fixed in 1.5% PFA, permeabilized in 90%, and 1 X 10e6 cells stained with 1.0 mcg/ml Phospho Zap70 (Y319) antibody in a 150 mcl volume. Isotype anti-KLH control (black) in H2O2 treated cells, anti-phospho Zap70 (Y319) in untreated cells (red), antiphospho Zap70 (Y319) in H2O2 treated (blue).