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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunoprecipitation [2]
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Validation data
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- Product number
- NBP1-18898 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-18898, RRID:AB_1625971
- Product name
- Rabbit Polyclonal ZAP70 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C. Do not freeze.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: ZAP70 [p Tyr494] Antibody [NBP1-18898] - Whole cell lysate (1 mg for IP, 20% of IP loaded) from Jurkat cells that had been treated with hydrogen peroxide (+) or mock treated (-). Antibodies: Affinity purified rabbit anti-ZAP70 antibody NB100-97826 was used at 3 mcg/mg lysate to immunoprecipitate ZAP70. The immunoprecipites were either treated with phosphatase (+) or mock (-) treated
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: ZAP70 [p Tyr494] Antibody [NBP1-18898] - Whole cell lysate (1 mg for IP, 20% of IP loaded) from Jurkat cells that had been treated with hydrogen peroxide. Antibodies: Affinity purified rabbit anti-Phospho ZAP70 antibodies NBP1-18896 (for phosphorylation at Y319), NBP1-18897 (for phosphorylation at Y493), and NBP1-18898 (for phosphorylation at Y493/Y494) used for IP at 3 mcg/mg lysate.
