Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- BS4178 - Provider product page
- Provider
- Bioworld Technology, Inc
- Proper citation
- Bioworld Technology Cat#BS4178, RRID:AB_1664101
- Product name
- Stat1 (phospho Y701) polyclonal antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic phosphopeptide derived from human Stat1 around the phosphorylation site of Tyrosine 701.
- Description
- Signal transducer and activator of transcription that mediates signaling by interferons (IFNs). Following type I IFN (IFN-alpha and IFN-beta) binding to cell surface receptors, Jak kinases (TYK2 and JAK1) are activated, leading to tyrosine phosphorylation of STAT1 and STAT2. The phosphorylated STATs dimerize, associate with ISGF3G/IRF-9 to form a complex termed ISGF3 transcription factor, that enters the nucleus. ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of interferon stimulated genes, which drive the cell in an antiviral state. In response to type II IFN (IFN-gamma), STAT1 is tyrosine- and serine-phosphorylated. It then forms a homodimer termed IFN-gamma-activated factor (GAF), migrates into the nucleus and binds to the IFN gamma activated sequence (GAS) to drive the expression of the target genes, inducing a cellular antiviral state.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100ul
- Concentration
- 1 mg/ml
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
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Supportive validation
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- Bioworld Technology, Inc (provider)
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- Experimental details
- Western blot (WB) analysis of p-Stat1 (Y701) pAb at 1:500Lane1: A549 cell lysate treated IFN-aLane2: DLD cell lysate treated IFN-aLane3: H9C2 cell lysate treated IFN-aLane4: Raw246.7 cell lysate treated IFN-aLane5: H9C2 cell lysate treated LPS