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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Flow cytometry [1]
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- Product number
- R30259 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- STAT1 Antibody
- Antibody type
- Polyclonal
- Description
- This highly specific STAT1 antibody is suitable for use in Western blot/Immunofluorescence/Flow cytometry applications with human, mouse and rat samples.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 100 ug
- Concentration
- 0.5mg/ml if reconstituted with 0.2ml sterile DI water
- Storage
- After reconstitution, the STAT1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of STAT1 antibody and human samples; Lane 1: MCF-7; 2: HeLa. Predicted molecular weight: 84 and 91 kDa.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of STAT1 antibody in mouse tissue; Lane 1: heart; 2: liver; 3: brain; 4: kidney; 5: spleen; 6: thymus; 7: lung; 8: intestine; 9: ovary
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of 1) human A549, 2) human HeLa, 3) human 293T, 4) human K562, 5) rat PC-12, 6) rat NRK and 7) mouse NIH 3T3 cell lysate with STAT1 antibody. Predicted molecular weight: ~91/84 kDa (alpha/beta).
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Immunofluorescent staining of FFPE human HeLa cells with STAT1 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Flow cytometry testing of fixed and permeabilized human A549 cells with STAT1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= STAT1 antibody.
