14-9008-82

antibody from Invitrogen Antibodies
Targeting: STAT1 ISGF-3, STAT91
Provider product page for 14-9008-82

Antibody data

Product number
14-9008-82 - Provider product page
Provider
Invitrogen Antibodies
Product name
Phospho-STAT1 (Tyr701) Monoclonal Antibody (KIKSI0803), eBioscience™
Antibody type
Monoclonal
Antigen
Other
Description
Description: This KIKSI0803 monoclonal antibody recognizes signal transducer and activator of transcription 1 (STAT1) when phosphorylated on tyrosine 701. STAT proteins are activated by ligand binding to receptors, such as cytokine receptors, that associate with Janus kinase (JAK) family members. Following their phosphorylation by JAKs, STAT proteins translocate to the nucleus where they bind to DNA and regulate transcription of specific genes in a cell type- and cytokine-specific manner. Phosphorylation of STAT1 on tyrosine 701 by JAK1 and JAK2 is essential for STAT1 dimer formation, nuclear translocation, and DNA binding activity. In response to IFN gamma stimulation, STAT1 homodimerizes or forms heterodimers with STAT3 that can bind to GAS (IFN gamma-activated sequence) promoter elements. In response to either IFN alpha or IFN beta stimulation, STAT1 forms a heterodimer with STAT2 that can bind ISRE (IFN-stimulated response element) promoter elements. Applications Reported: This KIKSI0803 antibody has been reported for use in intracellular staining followed by flow cytometric analysis, and western blotting. (Fluorochrome conjugated KIKSI0803 is recommended for use in intracellular flow cytometry.). Applications Tested: This KIKSI0803 antibody has been tested by immunoblotting of stimulated normal human peripheral blood cells. This can be used at less than or equal to 5 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 µm post-manufacturing filtered.
Reactivity
Human
Host
Mouse
Isotype
IgG
Antibody clone number
KIKSI0803
Vial size
100 µg
Concentration
0.5 mg/mL
Storage
4° C
STAT1 protein structure - 14-9008-82 shown in red.