14-9008-82

antibody from Invitrogen Antibodies

Targeting: STAT1 ISGF-3, STAT91

Provider product page for 14-9008-82

Western blot

Flow cytometry

Other assay

Antibody data

Antibody Data
Antigen structure
References [8]
Comments [0]
Validations
Western blot [1]
Other assay [3]

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Product number: 14-9008-82 - Provider product page
Provider: Invitrogen Antibodies
Product name: Phospho-STAT1 (Tyr701) Monoclonal Antibody (KIKSI0803), eBioscience™
Antibody type: Monoclonal
Antigen: Other
Description: Description: This KIKSI0803 monoclonal antibody recognizes signal transducer and activator of transcription 1 (STAT1) when phosphorylated on tyrosine 701. STAT proteins are activated by ligand binding to receptors, such as cytokine receptors, that associate with Janus kinase (JAK) family members. Following their phosphorylation by JAKs, STAT proteins translocate to the nucleus where they bind to DNA and regulate transcription of specific genes in a cell type- and cytokine-specific manner. Phosphorylation of STAT1 on tyrosine 701 by JAK1 and JAK2 is essential for STAT1 dimer formation, nuclear translocation, and DNA binding activity. In response to IFN gamma stimulation, STAT1 homodimerizes or forms heterodimers with STAT3 that can bind to GAS (IFN gamma-activated sequence) promoter elements. In response to either IFN alpha or IFN beta stimulation, STAT1 forms a heterodimer with STAT2 that can bind ISRE (IFN-stimulated response element) promoter elements. Applications Reported: This KIKSI0803 antibody has been reported for use in intracellular staining followed by flow cytometric analysis, and western blotting. (Fluorochrome conjugated KIKSI0803 is recommended for use in intracellular flow cytometry.). Applications Tested: This KIKSI0803 antibody has been tested by immunoblotting of stimulated normal human peripheral blood cells. This can be used at less than or equal to 5 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 µm post-manufacturing filtered.
Reactivity: Human
Host: Mouse
Isotype: IgG
Antibody clone number: KIKSI0803
Vial size: 100 µg
Concentration: 0.5 mg/mL
Storage: 4° C

STAT1 protein structure - 14-9008-82 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: NULL

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 4 Activation and differentiation markers in muscle regeneration. Densitometric analysis of Pax7 ( A ), Myf5 ( C ), MyoD ( D ), Myogenin ( F ), pSTAT3 ( G ) and pSTAT1 ( H ) protein expression levels in gastrocnemius. Data are normalized to endogenous control (alpha-tubulin) and expressed as arbitrary units (A.U.). Representative cropped blots of two animals of each group are shown, control and AiA, respectively. Full length blots are presented in Supplementary Figures 1 and 2 . Data represent individual values and medians with IQRs are also indicated (n = 6 rabbits per group). Representative TA cross sections with Pax7 ( B ) and MyoD ( E ) immunoreactive nuclei (black arrows) in control (left) and AiA (right) groups. Scale bar = 25 mum. Pax7 = paired box protein 7, Myf5 = myogenic factor 5, MyoD = myogenic differentiation 1, pSTAT = phosphorylated signal transducer and activator of transcription.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 1 ET HSCs Exhibit Prominent Mk Lineage Priming and Elevated Interferon (IFN) Signaling (A) Representative flow cytometry profiles for HSC isolation from bone marrow mononuclear cells (BM-MNCs) ( Table S1 ). (B) t-SNE analysis of transcriptomic profiles of HSCs from individuals with JAK2V617F + ET and PV and normal controls (NCs). (C) Selected feature genes from ET, PV, and NC HSCs. The percentage of expressing samples (Per.Exp) and average expression values (Aver.Exp) are shown. (D) GSEA of Mk-primed HSC gene sets in HSCs of affected individuals and NCs ( Table S2 ). Normalized enrichment score (NES), p value, and false discovery rate (FDR) are shown. (E) GSEA of various lineage-restricted or primed gene sets in pre-granulocytes and monocytes (pre-GMs), Mk and erythroid precursors (MegEs), and erythroid precursors (Erys) ( Table S2 ). (F) Strategy and representative flow cytometry of vWF + and CD41a + HSC subpopulations. (G) Proportions of vWF + and CD41a + HSC subpopulations shown in (F). (H) GSEA of the HALLMARK IFNalpha response gene set in HSCs of affected individuals and NCs. (I) STAT gene expression in ET, PV, and NC HSCs. Log 2 -transformed (fragments per kilobase million [FPKM]+1) are shown for individuals. Thick horizontal lines indicate median expression levels, and boxes represent the first and third quartiles. (J) Strategy and representative flow cytometry for measurement of phosphorylated STAT1 (p-STAT1), p-STAT3, and p-STAT5 levels. (K) Relative fluoresce