48-9008-42

antibody from Invitrogen Antibodies

Targeting: STAT1 ISGF-3, STAT91

Flow cytometry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

48-9008-42

Provider

Invitrogen Antibodies

Product name

Phospho-STAT1 (Tyr701) Monoclonal Antibody (KIKSI0803), eFluor™ 450, eBioscience™

Antibody type

Monoclonal

Antigen

Other

Description

Description: This KIKSI0803 monoclonal antibody recognizes signal transducer and activator of transcription 1 (STAT1) when phosphorylated on tyrosine 701. STAT proteins are activated by ligand binding to receptors, such as cytokine receptors, that associate with Janus kinase (JAK) family members. Following their phosphorylation by JAKs, STAT proteins translocate to the nucleus where they bind to DNA and regulate transcription of specific genes in a cell type- and cytokine-specific manner. Phosphorylation of STAT1 on tyrosine 701 by JAK1 and JAK2 is essential for STAT1 dimer formation, nuclear translocation, and DNA binding activity. In response to IFN gamma stimulation, STAT1 homodimerizes or forms heterodimers with STAT3 that can bind to GAS (IFN gamma-activated sequence) promoter elements. In response to either IFN alpha or IFN beta stimulation, STAT1 forms a heterodimer with STAT2 that can bind ISRE (IFN-stimulated response element) promoter elements.

Antibody clone number

KIKSI0803

Concentration

5 µL/Test

References

Submitted references

Hematopoietic stem cell heterogeneity is linked to the initiation and therapeutic response of myeloproliferative neoplasms.

Tong J, Sun T, Ma S, Zhao Y, Ju M, Gao Y, Zhu P, Tan P, Fu R, Zhang A, Wang D, Wang D, Xiao Z, Zhou J, Yang R, Loughran SJ, Li J, Green AR, Bresnick EH, Wang D, Cheng T, Zhang L, Shi L
Cell stem cell 2021 Apr 1;28(4):780

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Proteomic Analyses of Human Regulatory T Cells Reveal Adaptations in Signaling Pathways that Protect Cellular Identity.

Cuadrado E, van den Biggelaar M, de Kivit S, Chen YY, Slot M, Doubal I, Meijer A, van Lier RAW, Borst J, Amsen D
Immunity 2018 May 15;48(5):1046-1059.e6

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Immune-Mediated Inflammation May Contribute to the Pathogenesis of Cardiovascular Disease in Mucopolysaccharidosis Type I.

Khalid O, Vera MU, Gordts PL, Ellinwood NM, Schwartz PH, Dickson PI, Esko JD, Wang RY
PloS one 2016;11(3):e0150850

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Intracellular staining of unstimulated (orange histogram) or 15-minute IFN gamma-stimulated (purple histogram) normal human peripheral blood cells with Anti-Human phospho-STAT1 (Y701) eFluor® 450 using the Fixation/Methanol Protocol. CD4-low monocytes were used for analysis.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

NULL

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 1 ET HSCs Exhibit Prominent Mk Lineage Priming and Elevated Interferon (IFN) Signaling (A) Representative flow cytometry profiles for HSC isolation from bone marrow mononuclear cells (BM-MNCs) ( Table S1 ). (B) t-SNE analysis of transcriptomic profiles of HSCs from individuals with JAK2V617F + ET and PV and normal controls (NCs). (C) Selected feature genes from ET, PV, and NC HSCs. The percentage of expressing samples (Per.Exp) and average expression values (Aver.Exp) are shown. (D) GSEA of Mk-primed HSC gene sets in HSCs of affected individuals and NCs ( Table S2 ). Normalized enrichment score (NES), p value, and false discovery rate (FDR) are shown. (E) GSEA of various lineage-restricted or primed gene sets in pre-granulocytes and monocytes (pre-GMs), Mk and erythroid precursors (MegEs), and erythroid precursors (Erys) ( Table S2 ). (F) Strategy and representative flow cytometry of vWF + and CD41a + HSC subpopulations. (G) Proportions of vWF + and CD41a + HSC subpopulations shown in (F). (H) GSEA of the HALLMARK IFNalpha response gene set in HSCs of affected individuals and NCs. (I) STAT gene expression in ET, PV, and NC HSCs. Log 2 -transformed (fragments per kilobase million [FPKM]+1) are shown for individuals. Thick horizontal lines indicate median expression levels, and boxes represent the first and third quartiles. (J) Strategy and representative flow cytometry for measurement of phosphorylated STAT1 (p-STAT1), p-STAT3, and p-STAT5 levels. (K) Relative fluoresce