M00036-2

antibody from Boster Biological Technology

Targeting: STAT1 ISGF-3, STAT91

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Data presented on provider website)

Flow cytometry (Data presented on provider website)

Antibody Data

Product number

M00036-2

Provider

Boster Biological Technology

Product name

Anti-STAT1 Antibody Picoband™ (monoclonal, 12C7)

Antibody type

Monoclonal

Description

Mouse IgG monoclonal antibody for STAT1 detection. Tested with WB, IHC-P, ICC/IF, FCM in Human;Monkey.

Reactivity

Human, Simian

Host

Mouse

Isotype

IgG

Antibody clone number

12C7

Vial size

100μg/vial

Concentration

0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Storage

At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquoted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.

Handling

Add 0.2ml of distilled water will yield a concentration of 500μg/ml.

References

Submitted references

Blockage of Glyoxalase I Inhibits Colorectal Tumorigenesis and Tumor Growth via Upregulation of STAT1, p53, and Bax and Downregulation of c-Myc and Bcl-2.

Chen Y, Fang L, Zhang J, Li G, Ma M, Li C, Lyu J, Meng QH
International journal of molecular sciences 2017 Mar 9;18(3)

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Death signal transduction induced by co-immobilized TNF-α plus IFN-γ and the development of polymeric anti-cancer drugs.

Guan YQ, Li Z, Liu JM
Biomaterials 2010 Dec;31(34):9074-85

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Effects of acitretin on proliferative inhibition and RANTES production of HaCaT cells.

Zhang M, Zhu L, Feng Y, Yang Y, Liu L, Ran Y
Archives of dermatological research 2008 Nov;300(10):575-81

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Hepatitis C virus non-structural 5A abrogates signal transducer and activator of transcription-1 nuclear translocation induced by IFN-alpha through dephosphorylation.

Gong GZ, Cao J, Jiang YF, Zhou Y, Liu B
World journal of gastroenterology 2007 Aug 14;13(30):4080-4

Western blot

Supportive validation

Submitted by

Boster Biological Technology (provider)

Main image

Experimental details

Western blot analysis of STAT1 using anti-STAT1 antibody (M00036-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human placenta tissue lysates, Lane 3: human 22RV1 whole cell lysates. Lane 4: monkey COS-7 whole cell lysates, Lane 5: human SW620 whole cell lysates, Lane 6: human HepG2 whole cell lysates, Lane 7: human PANC-1 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-STAT1 antigen affinity purified monoclonal antibody (Catalog # M00036-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for STAT1 at approximately 87KD. The expected band size for STAT1 is at 87KD.

Additional image