HPA000982

antibody from Atlas Antibodies

Targeting: STAT1 ISGF-3, STAT91

Western blot (Data presented on provider website)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Data presented on provider website)

Chromatin Immunoprecipitation (Supportive data in Antibodypedia)

Antibody Data

Product number

HPA000982

Provider

Atlas Antibodies

Proper citation

Atlas Antibodies Cat#HPA000982, RRID:AB_1080099

Product name

Anti-STAT1

Antibody type

Polyclonal

Description

Polyclonal Antibody against Human STAT1, Gene description: signal transducer and activator of transcription 1, 91kDa, Alternative Gene Names: ISGF-3, STAT91, Validated applications: ChIP, ICC, WB, IHC, Uniprot ID: P42224, Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Conjugate

Unconjugated

Isotype

IgG

Vial size

100 µl

Concentration

0.2 mg/ml

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C.

Handling

The antibody solution should be gently mixed before use.

References

Submitted references

The Foot-and-Mouth Disease Virus Lb Protease Cleaves Intracellular Transcription Factors STAT1 and STAT2 to Antagonize IFN-β–Induced Signaling

Ma X, Luo Z, Song R, Nian X, Choudhury S, Ru Y, Yang F, Zhang Y, Zeng Z, Cao W, Pei J, Liu X, Zheng H
The Journal of Immunology 2023;210(3):283-296

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Aggressive PDACs Show Hypomethylation of Repetitive Elements and the Execution of an Intrinsic IFN Program Linked to a Ductal Cell of Origin

Espinet E, Gu Z, Imbusch C, Giese N, Büscher M, Safavi M, Weisenburger S, Klein C, Vogel V, Falcone M, Insua-Rodríguez J, Reitberger M, Thiel V, Kossi S, Muckenhuber A, Sarai K, Lee A, Backx E, Zarei S, Gaida M, Rodríguez-Paredes M, Donato E, Yen H, Eils R, Schlesner M, Pfarr N, Hackert T, Plass C, Brors B, Steiger K, Weichenhan D, Arda H, Rooman I, Kopp J, Strobel O, Weichert W, Sprick M, Trumpp A
Cancer Discovery 2021;11(3):638-659

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STAT1 as a potential prognosis marker for poor outcomes of early stage colorectal cancer with microsatellite instability

de Miranda N, Tanaka A, Zhou Y, Ogawa M, Shia J, Klimstra D, Wang J, Roehrl M
PLOS ONE 2020;15(4):e0229252

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The Vaccinia Virus (VACV) B1 and Cellular VRK2 Kinases Promote VACV Replication Factory Formation through Phosphorylation-Dependent Inhibition of VACV B12

Rico A, Wang Z, Olson A, Linville A, Bullard B, Weaver E, Jones C, Wiebe M, Shisler J
Journal of Virology 2019;93(20)

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A High-throughput Bead-based Affinity Assay Enables Analysis of Genital Protein Signatures in Women At Risk of HIV Infection

Månberg A, Bradley F, Qundos U, Guthrie B, Birse K, Noël-Romas L, Lindskog C, Bosire R, Kiarie J, Farquhar C, Burgener A, Nilsson P, Broliden K
Molecular & Cellular Proteomics 2019;18(3):461-476

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Silvestrol Inhibits Chikungunya Virus Replication

Henss L, Scholz T, Grünweller A, Schnierle B
Viruses 2018;10(11):592

Chromatin Immunoprecipitation

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

ChIP-Exo-Seq composite graph for Anti-STAT1 (HPA000982, Lot 000053776) tested in K562 cells. Strand-specific reads (blue: forward, red: reverse) and IgG controls (black: forward, grey: reverse) are plotted against the distance from a composite set of reference binding sites. The antibody exhibits robust target enrichment compared to a non-specific IgG control and precisely reveals its structural organization around the binding site. Data generated by Prof. B. F. Pugh´s Lab at Cornell University.