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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
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- Product number
- MA1-19323 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- STAT1 Monoclonal Antibody (SM1)
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide
- Description
- This antibody recognizes an epitope included within amino acids 721-733 of STAT1, a 91 kDa transcriptional factor involved in a variety of systems including antiviral responses and interferon alpha (IFN-alpha) and gamma (IFN-gamma) signal transduction.
- Reactivity
- Human, Mouse
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- SM1
- Vial size
- 100 µg
- Concentration
- 1 mg/mL
- Storage
- 4° C, do not freeze
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of STAT1 using a monoclonal antibody (Product # MA1-19323).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blotting analysis of human STAT1 using mouse monoclonal antibody SM1 on lysates of HeLa, MCF-7, and Cos-7 cell lines under reducing conditions. Nitrocellulose membrane was probed with 2µg/mL of mouse Monoclonal antibody anti-STAT1 (Product # MA1-19323) followed by IRDye800-conjugated anti-mouse secondary antibody. STAT1 was detected at approximately 90kDa.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of Human phosphorylated STAT1[p Ser727] and total STAT1 by Western Blot.Western blot shows lysate of human malignant melanoma cells (short-term culture derived from a patient) in response to interferon treatment to induce phosphorylation of STAT1 at Ser727. Subconfluent cells were serum-starved before exposure to activation dosages of IFN-gamma (10 ng/mL) and IFN-alpha (1000 IU/mL and 5000 IU/mL). Specific band was detected for phosphorylated STAT1[Ser727] by the antibody PSM1 (upper panel) and total STAT1 level by SM1 Monoclonal antibody (Product # MA1-19323) (lower panel).
