MA5-15071

antibody from Invitrogen Antibodies

Targeting: STAT1 ISGF-3, STAT91

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-15071

Provider

Invitrogen Antibodies

Product name

Phospho-STAT1 (Tyr701) Monoclonal Antibody (S.213.5)

Antibody type

Monoclonal

Antigen

Synthetic peptide

Description

It is not recommended to aliquot this antibody.

Antibody clone number

S.213.5

Concentration

59 µg/mL

References

Submitted references

An organoid-derived bronchioalveolar model for SARS-CoV-2 infection of human alveolar type II-like cells.

Lamers MM, van der Vaart J, Knoops K, Riesebosch S, Breugem TI, Mykytyn AZ, Beumer J, Schipper D, Bezstarosti K, Koopman CD, Groen N, Ravelli RBG, Duimel HQ, Demmers JAA, Verjans GMGM, Koopmans MPG, Muraro MJ, Peters PJ, Clevers H, Haagmans BL
The EMBO journal 2021 Mar 1;40(5):e105912

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Sustained Inflammatory Signalling through Stat1/Stat2/IRF9 Is Associated with Amoeboid Phenotype of Melanoma Cells.

Gandalovičová A, Šůchová AM, Čermák V, Merta L, Rösel D, Brábek J
Cancers 2020 Aug 28;12(9)

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MicroRNA-144 Regulates Cardiomyocyte Proliferation and Apoptosis by Targeting TBX1 through the JAK2/STAT1 Pathway.

Cao ML, Zhu BL, Sun YY, Qiu GR, Fu WN, Jiang HK
Cytogenetic and genome research 2019;159(4):190-200

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Infectious Bronchitis Coronavirus Inhibits STAT1 Signaling and Requires Accessory Proteins for Resistance to Type I Interferon Activity.

Kint J, Dickhout A, Kutter J, Maier HJ, Britton P, Koumans J, Pijlman GP, Fros JJ, Wiegertjes GF, Forlenza M
Journal of virology 2015 Dec;89(23):12047-57

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of Phospho-STAT1 (Tyr701) using a monoclonal antibody (Product # MA5-15071).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of Phospho-STAT1 (Tyr701) using a monoclonal antibody (Product # MA5-15071).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of Phospho-STAT1 (Tyr701) using a monoclonal antibody (Product # MA5-15071).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of Phospho-STAT1 (Tyr701) using a monoclonal antibody (Product # MA5-15071).

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry analysis of Phospho-STAT1 (Tyr701) using a monoclonal antibody (Product # MA5-15071).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

NULL

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

6 Figure Host response analysis of SARS-CoV-2 infection in organoid-derived bronchioalveolar-like and small airway cells pSTAT1 translocation in mock and SARS-CoV-2-infected bronchioalveolar-like cells visualized in green. Scale bars indicate 50 mum. Nucleoprotein (NP) stains viral capsid (red). Nuclei are stained with Hoechst (white). Scale bar indicates 50 um. Heatmaps depicting top 20 enriched genes based on adjusted P -value in bronchioalveolar-like cells after SARS-CoV-2 infection. Colored bars represent Z -scores of log 2 -transformed values. pSTAT1 translocation in mock and SARS-CoV-2-infected small airway cells visualized in green. Nucleoprotein (NP) stains viral capsid (red). Nuclei are stained with Hoechst (white). Scale bar indicates 50 mum. Heatmaps depicting top 25 enriched genes based on adjusted p -value and log2 fold changes in small airway cells after SARS-CoV-2 infection. Colored bars represent Z -scores of log 2 -transformed values. GO term enrichment analysis for biological processes of the 20 most significantly up-regulated genes upon SARS-CoV-2 infected in bronchioalveolar-like cells. P -values were determined using PANTHER Overrepresentation Test. GO term enrichment analysis for biological processes of the 25 most significantly up-regulated genes upon SARS-CoV-2 infected in small airway cells. P -values were determined using PANTHER Overrepresentation Test.