PAB20073

antibody from Abnova Corporation

Targeting: DNMT3B

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB20073

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#PAB20073, RRID:AB_10965482

Product name

DNMT3B polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against recombinant DNMT3B.

Antigen sequence

MEPSPEPPSLESMKGDTRHLNGEEDAGGREDSILV
NGACSDQSSDSPPILEAIRTPEIRGRRSSSRLSKR
EVSSLLSYTQDLTGDGDGEDGDGSDTPVMPKLFRE
TRTRSESPAVRTRNNNSVSSRERHRPSPRSTRGRQ
GRNHVDES


Isotype

IgG

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of Lane 1: RT-4, Lane 2: U-251 MG, Lane 3: Human Plasma, Lane 4: Liver, Lane 5: Tonsil with DNMT3B polyclonal antibody (Cat # PAB20073) at 1:250-1:500 dilution.

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line U-2 OS with DNMT3B polyclonal antibody (Cat # PAB20073) at 1-4 ug/mL dilution shows positivity in nucleus but not nucleoli, vesicles.

Validation comment

Immunofluorescence

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunohistochemical staining of human testis with DNMT3B polyclonal antibody (Cat # PAB20073) shows strong nuclear positivity in cells of seminiferus ducts at 1:50-1:200 dilution.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)