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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [4]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Other assay [2]
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- Product number
- PA5-85549 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- DNMT3B Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Keep as concentrated solution. Predicted reactivity: Pig (85%). Positive Control: HeLa, HeLa nuclear, GFP-tagged DNMT3B-transfested 293T. Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1.29 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references PROSER1 mediates TET2 O-GlcNAcylation to regulate DNA demethylation on UTX-dependent enhancers and CpG islands.
Wang X, Rosikiewicz W, Sedkov Y, Martinez T, Hansen BS, Schreiner P, Christensen J, Xu B, Pruett-Miller SM, Helin K, Herz HM
Life science alliance 2022 Jan;5(1)
Life science alliance 2022 Jan;5(1)
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of DNMT3B in wild-type (WT) and DNMT3B knockout (KO) HeLa cell extracts using DNMT3B polyclonal antibody (Product # PA5-85549) using 30 µg of sample at a dilution of 1:500. Sample was then incubated with HRP-conjugated anti-rabbit IgG secondary antibody. Prior to incubation with primary antibody, the sample was separated on 7.5% SDS-PAGE.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of DNMT3B in HeLa whole cell extracts and nuclear extracts using DNMT3B polyclonal antibody (Product # PA5-85549) using 30 µg of sample at a dilution of 1:500. Prior to incubation with primary antibody, the sample was separated on 5% SDS-PAGE.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of DNMT3B was performed by separating 30 µg of non-transfected (–) and transfected (+) 293T whole cell extracts by 5% SDS-PAGE. Proteins were transferred to a membrane and probed with a DNMT3B Polyclonal Antibody (Product # PA5-85549) at a dilution of 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody., and the signal was developed with Trident ECL plus-Enhanced
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- DNMT3B Polyclonal Antibody detects DNMT3B protein by western blot analysis. HeLa whole cell extracts and nuclear extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with DNMT3B Polyclonal Antibody (Product # PA5-85549) diluted by 1:500.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- DNMT3B Polyclonal Antibody detects DNMT3B protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: DNMT3B protein stained by DNMT3B Polyclonal Antibody (Product # PA5-85549) diluted at 1:200. Red: phalloidin, a cytoskeleton marker, diluted at 1:50. Scale bar = 10 µm.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- DNMT3B Polyclonal Antibody detects DNMT3B protein at nucleus on human lung carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human lung carcinoma. DNMT3B Polyclonal Antibody (Product # PA5-85549) diluted at 1:250. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation analysis of DNMT3B in K562 nuclear extracts with DNMT3B polyclonal antibody (Product # PA5-85549) using 5 µg of sample, followed by anti-Rabbit IgG secondary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure S7. Exploring alternative mechanisms of PROSER1-mediated changes in DNA methylation. (A) Western blot for members of the DNA methyltransferase family from nuclear extracts of WT and PROSER1 KO HEK293 cells. Actin was used as a loading control. (B) DNA methylation rank plots showing regions associated with TET1 down-regulation, TET2 down-regulation, TET1 up-regulation, TET2 up-regulation, UTX/H3K4me1 down-regulation, and PROSER1-occupied regions in PROSER1 KO versus WT HEK293 cells (all FC >= 2). Regions with CpGs covered by at least 10 whole genome bisulfite sequencing reads (please see ""whole genome bisulfite sequencing data processing"" in the ""Materials and Methods"" section for further details) were ranked from hyper- to hypomethylated on the x-axis.
