600-401-485

antibody from Rockland Immunochemicals, Inc.

Targeting: FBXO43 Fbx43

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

600-401-485

Provider

Rockland Immunochemicals, Inc.

Proper citation

Rockland Cat#600-401-485, RRID:AB_828154

Product name

Anti-F-Box Protein 43 (Fbp5B) (RABBIT) Antibody - 600-401-485

Antibody type

Polyclonal

Vial size

100 µl

Western blot

Supportive validation

Submitted by

Rockland Immunochemicals, Inc. (provider)

Main image

Experimental details

Western blot using Rockland's affinity purified anti-Fbp5B antibody. Panels A and B show two identical membranes containing marker, in vitro translated Fbp5b (Fbp5b ivt), and a whole cell extract from 293T cells loaded in duplicate). Panel A shows antibody reactivity. Panel B shows antibody reactivity after first pre-incubating the antibody with the immunizing peptide. This pre-incubation greatly diminishes specific band recognition. Peptide inhibition completely removes the in vitro translated band and greatly reduces the endogenous band from 293T cells. When this experiment was repeated using a mock in vitro translation, no band was detected (data not shown). Personal Communication, Daniele Guardavaccaro, NYU Cancer Institute

Validation comment

Western Blot