Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [2]
- Immunohistochemistry [2]
- Other assay [1]
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- Product number
- PA5-21622 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- FBXO43 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references A Novel Signature of CCNF-Associated E3 Ligases Collaborate and Counter Each Other in Breast Cancer.
Chang SC, Hung CS, Zhang BX, Hsieh TH, Hsu W, Ding JL
Cancers 2021 Jun 8;13(12)
Cancers 2021 Jun 8;13(12)
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using FBXO43 Polyclonal Antibody (Product # PA5-21622). Sample (30 µg of whole cell lysate). Lane A: NIH-3T3. Lane B: JC . 7.5% SDS PAGE. FBXO43 Polyclonal Antibody (Product # PA5-21622) diluted at 1:1,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using FBXO43 Polyclonal Antibody (Product # PA5-21622). Sample (30 µg of whole cell lysate). Lane A: 293T . 7.5% SDS PAGE. FBXO43 Polyclonal Antibody (Product # PA5-21622) diluted at 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Fbp5B in paraformaldehyde-fixed A431 cells using a Fbp5B polyclonal antibody (Product # PA5-21622) at a 1:200 dilution.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of paraformaldehyde-fixed A431, using FBXO43 antibody (Product # PA5-21622) at 1:200 dilution.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of FBXO43 was performed in paraffin-embedded TOV-21G xenograft tissue using FBXO43 Polyclonal Antibody (Product # PA5-21622) at a dilution of 1:250.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded Saos-2 xenograft, using FBXO43 (Product # PA5-21622) antibody at 1:100 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 2 Transcriptional profiles of the main components of SCF E3 from the TCGA database of human primary BRCA tissues. To clarify the potential of the identified SCF E3 ligase, both TCGA database results ( A - C ) and IHC results ( D ) were analyzed. ( A ) As a key E1 component, UBA1 was elevated in BRCA tissues throughout all disease stages. ( B ) UBE2C, UBE2T, and UBE2O were identified as the main E2 components, showing significant overexpression in breast carcinoma tissues, particularly in advanced stages. ( C ) Investigation of FBXL8 profiling was recently studied in BRCA tissues []. Here, we further reported that CCNF, FBXO43, and FBXO15 were identified as the main E3 F-box components, which show significant overexpression in carcinoma tissues. Y-axis: normalized count, X-axis: pathological cancer stages. The number of samples in each stage is shown in parentheses: stage I/0 ( n = 182), stage II ( n = 624), stage III ( n = 251), and stage IV ( n = 20). N: normal ( n = 113). *, p < 0.05; **, p < 0.01. ( D ) shows H&E and immunofluorescence staining. Protein expression levels of CCNF, FBXO43, and FBXO15 were examined in both breast carcinoma ( n = 30) and paired normal breast tissues ( n = 30). The corresponding quantitative results of CCNF, FBXO43, and FBXO15 in IHC are shown in ( E - G ), respectively. Consistent with our observation of the mRNA levels, the FBXL8 [], FBXO43, and FBXO15 proteins are significantly upregulated in breast carcinoma tissues compared with nor