MA1-40155

antibody from Invitrogen Antibodies

Targeting: C3 ARMD9, C3a, C3b, CPAMD1

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

MA1-40155

Provider

Invitrogen Antibodies

Product name

Complement C3b Monoclonal Antibody (755)

Antibody type

Monoclonal

Antigen

Other

Description

MA1-40155 detects C3/C3b from human samples. MA1-40155 has been successfully used in ELISA and Western blot applications. In western blot, the band size is ~190 kDa under non-reducing and ~100 kDa under reducing conditions. The MA1-40155 immunogen is native C3. Positive control: Human serum; Negative control: C3 deficient serum

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

755

Vial size

100 µg

Concentration

0.1 mg/mL

Storage

4° C

References

Submitted references

Complement-Mediated Differential Immune Response of Human Macrophages to Sporothrix Species Through Interaction With Their Cell Wall Peptidorhamnomannans.

Neves GWP, Wong SSW, Aimanianda V, Simenel C, Guijarro JI, Walls C, Willment JA, Gow NAR, Munro CA, Brown GD, Lopes-Bezerra LM
Frontiers in immunology 2021;12:749074

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Differential Interactions of Serum and Bronchoalveolar Lavage Fluid Complement Proteins with Conidia of Airborne Fungal Pathogen Aspergillus fumigatus.

Wong SSW, Daniel I, Gangneux JP, Jayapal JM, Guegan H, Dellière S, Lalitha P, Shende R, Madan T, Bayry J, Guijarro JI, Kuppamuthu D, Aimanianda V
Infection and immunity 2020 Aug 19;88(9)

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot was performed using Anti-Complement C3b Monoclonal Antibody (755) (Product # MA1-40155) and a 187 kDa band corresponding to Complement C3 was observed in HepG2 in comparison to HEK-293 which is reported to be negative. A band around 70 kDa was also observed in Hep G2 treated with LPS and PTI which can be the processed form of complement C3. Whole cell extracts (30 µg lysate) of Hep G2 (Lane 1), Hep G2 treated with LPS (10 ng/mL for 18 hr) followed by protein transport inhibitor (1X for 4 hr) (Lane 2) and HEK-293 (Lane 3) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:50 dilution) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using SuperSignal™ West Dura Extended Duration Substrate (Product # 34076).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 7 Interaction between complement system and peptidorhamnomannans (PRMs) of Sporothrix species. (A) Sporothrix yeasts were opsonized with C3b and labeled with mouse anti-C3b mAb and mouse TRITC-IgG (Ab). PRMs (50 ug/ml in 50 mM carbonate buffer, pH 9.6) were coated on 96-well plate: their (B, C) C3 activation capacities were determined using GVB+ or GVB- (with EGTA) media using whole/complement factor-depleted human sera. (D ) Interaction with CD11b was determined by sequential addition of CD11b, mouse anti-CD11b mAb, peroxidase-conjugated mouse IgG, and chromogenic substrate for peroxidase (tetramethyl benzidine); colors developed were optically read at 450 nm. For each condition, two independent experiments, in triplicate, were performed. Statistical analysis was performed by ANOVA with Tukey's multiple comparison posttest (****p < 0.0001).