MA1-40158

antibody from Invitrogen Antibodies

Targeting: C3 ARMD9, C3a, C3b, CPAMD1

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Antibody Data

Product number

MA1-40158

Provider

Invitrogen Antibodies

Product name

Complement C3a Monoclonal Antibody (2898)

Antibody type

Monoclonal

Antigen

Other

Description

MA1-40158 detects C3/C3a from human samples. MA1-40158 has been successfully used in Western blot and ELISA applications. The MA1-40158 immunogen is the C-terminus of C3. Positive control: Human plasma; Negative control: C3-depleted serum

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

2898

Vial size

100 µg

Concentration

0.1 mg/mL

Storage

4° C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot was performed using Anti-Complement C3a Monoclonal Antibody (2898) (Product # MA1-40158) and a 187 kDa band corresponding to Complement C3 was observed in HepG2 in comparison to HEK-293 which is reported to be negative. A band around 120 kDa was also observed in Hep G2 treated with LPS and PTI which can be the processed form of complement C3. Whole cell extracts (30 µg lysate) of Hep G2 (Lane 1), Hep G2 treated with protein transport inhibitor (1X for 4 hr) (Lane 2) and Hep G2 treated with LPS (10 ng/mL for 18 hr) followed by protein transport inhibitor (1X for 4 hr) (Lane 3), HEK-293 (Lane 4) and HEK-293 treated with protein transport inhibitor (1X for 4 hr) (Lane 5) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:50 dilution) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Reagent Kit (Product # WP20005).