846106

antibody from BioLegend
Targeting: C3 ARMD9, C3a, C3b, CPAMD1
Provider product page for 846106

Antibody data

Product number
846106 - Provider product page
Provider
BioLegend
Proper citation
BioLegend Cat#846106, RRID:AB_2632795
Product name
APC anti-complement C3b/iC3b
Antibody type
Monoclonal
Description
Complement C3 is a 185 kD glycoprotein composed of two chains linked by a disulfide bond. It is the fourth complement component to react in the classical pathway. It is also a key protein in both the alternative and the lectin complement activation pathways. C3b is proteolytically generated from C3 by cleavage of the C3a-C3b peptide bond in the protein by C3 convertase. C3b can bind covalently, via its reactive thioester, to cell surface carbohydrates or immune aggregates. Macrophages and neutrophils recognize C3b by the complement receptor 1 (CR1, CD35). Opsonization of target surfaces with C3b is central to all three pathways of complement activation. The proteolytically inactive forms of C3b, iC3b, are generated by cleavage of the alpha chain at one or two positions by factor I in the presence of co-factors, such as factor H.  A fragment of C3b, C3f, with a molecular weight of approximately 2 kD is generated when C3b is cleaved at two positions by factor I. Although iC3b is less active than C3b, iC3b’s interactions with lymphoid and phagocytic cells via CR2 (CD21) and CR3 (CD11b/CD18) can expand target-specific B and T cells. iC3b can be cleaved to form C3c and C3dg.
Reactivity
Human
Host
Mouse
Conjugate
Red dye
Antibody clone number
3E7/C3b
Vial size
100 tests
Storage
2°C-8°C
Handling
Ambient RT
C3 protein structure - 846106 shown in red.