518002

antibody from BioLegend

Targeting: C3 ARMD9, C3a, C3b, CPAMD1

Western blot (Recommended by provider)

ELISA (Data presented on provider website)

Antibody Data

Product number

518002

Provider

BioLegend

Proper citation

BioLegend Cat#518002, RRID:AB_10895750

Product name

Biotin anti-human C3a/C3a(desArg)/C3

Antibody type

Monoclonal

Antigen

Human C3a

Description

C3, complement component 3, is a 195 KD glycoprotein heterodimer that is linked by disulfide bonds. It is the fourth complement component to react in the classical pathway and it is also a reactant in the alternative complement pathway. C3 convertase splits C3 into C3b and C3a. C3b can bind covalently, via its reactive thioester, to cell surface carbohydrates or immune aggregates. Macrophages and neutrophils have receptors for C3b. C3a is released into the surrounding fluids. It can bind to receptors on basophils and mast cells triggering them to release their vasoactive contents (e.g., histamine). Because of the role of these materials in anaphylaxis, C3a is called an anaphylatoxin. In blood, the C3a fragment is rapidly desarginated by serum carboxypeptidase N enzyme to generate C3a desArg. Both C3a and C3a desArg are not glycosylated.

Reactivity

Human

Host

Mouse

Conjugate

Biotin

Isotype

IgG

Vial size

500 µg

Concentration

0.5 mg/ml

Storage

The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.