Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
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Validation data
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- Product number
- PA5-76234 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- C4BPA Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
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- Experimental details
- Western blot analysis of C4BPA in extracts of various cells. Samples were incubated with C4BPA polyclonal antibody (Product # PA5-76234).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-C4BPA Polyclonal Antibody (Product # PA5-76234) and bands corresponding to 93kDa and 52kDa of C4BPA was observed in the reported positive models- Hep G2 and Mouse Epididymis relative to DU 145, BeWO and Mouse Lung which are low expressing models along with an uncharacterized band (*) at ~40kDa in the cell lines tested. Whole cell extracts (30 µg lysate) of Hep G2 (Lane 1), DU 145 (Lane 2), BeWO (Lane 3), tissue extracts (30ug lysate) of Mouse Lungs (Lane 4) and Mouse Epididymis (Lane 5) were electrophoresed using NuPAGE™ 10% Bis-Tris Protein Gel (Product # NP0302BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001). The blot was probed with the antibody (1:1200 dilution) and detected by ECL with Goat anti-Rabbit IgG (H+L), Superclonal™ secondary, HRP (Product # A27036, 1:4000 dilution). ECL was performed using Novex® ECL Substrate Reagent Kit (Product # WP20005). C4 protein, composed of alpha, beta and gamma chain, forms a heterotrimer of ~93 kDa alpha chains via disulphide linkage as detected by this antibody (10.4049/jimmunol.165.5.2518) and a ~52 kDa protein corresponding to mouse and rat specific isoforms of C4BPA. It is also reported to be secreted by the epithelial cells surrounding the epididymis for sperm maturation in a region-specific, androgen dependent and species specific manner in addition to being synthesized in the liver.