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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [2]
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- Product number
- PA5-96371 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Complement Factor I Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: KVTYTSQEDL VEKKCLAKKY THLSCDKVFC QPWQRCIEGT CVCKLPYQCP KNGTAVCATN RRSFPTYCQQ KSLECLHPGT KFLNNGTCTA EGKFSVSLKH GNTDSEGIVE VKLVDQDKTM FICKSSWSMR EANVACLDLG FQQGADTQRR FKLSDLSINS TECLHVHCRG LETSLAECTF TKRRTMGYQD FADVVCYTQK ADSPMDDFFQ CVNGKYISQM KACDGINDCG DQSDELCCKA CQGKGFHCKS GVCIPSQYQC NGEVDCITGE DEVGCAGFAS VA; Positive Samples: Mouse liver, Mouse intestine; Cellular Location: extracellular space
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.27 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
Submitted references Isolation, culture, and cryosectioning of primary porcine retinal pigment epithelium on transwell cell culture inserts.
Hood EMS, Curcio CA, Lipinski D
STAR protocols 2022 Dec 16;3(4):101758
STAR protocols 2022 Dec 16;3(4):101758
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of extracts of various cell lines, using CFI Polyclonal antibody (Product # PA5-96371) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Exposure time: 90s.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of Complement Factor I in extracts of Human serum using Complement Factor I Polyclonal Antibody (Product # PA5-96371) at a dilution of 1:1000. A HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
