LS-C344799

antibody from LSBio

Targeting: CASP8 Casp-8, FLICE, MACH, MCH5

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunocytochemistry (Recommended by provider)

Immunohistochemistry (Recommended by provider)

Antibody Data

Product number

LS-C344799

Provider

LSBio

Product name

CASP8 / Caspase 8 Antibody (clone 3B10) LS-C344799

Antibody type

Monoclonal

Description

Protein G purified from tissue culture supernatant

Reactivity

Mouse

Host

Rat

Isotype

IgG

Antibody clone number

3B10

Storage

Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Western blot using anti-Caspase-8 (mouse), mAb (3B10) detecting endogenous caspase-8 in various mouse cell line, but not in human cell lines.

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Western blot using anti-Caspase-8 (mouse), mAb (3B10) detecting the cleaved active p20 subunit of mouse caspase-8 in addition to the caspase-8 precursor, upon an apoptotic stimulus e.g. by cross-linked rhsFasL.

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Western blot using anti-Caspase-8 (mouse), mAb (3B10) detecting endogenous caspase-8 in MEFs from WT mice, but not in MEFs from caspase-8-/- mice. Several smaller bands detected in the caspase-8-/- MEFs, correspond to truncated forms of caspase-8 made in the caspase-8-/- mice since only exons 1 and 2 of mouse caspase-8 were deleted in these knock-out mice and not the region encoding the p20 subunit. Note: Extra bands marked by * are only seen in lysates from caspase-8-/- MEFs and not in lysates from any WT cell lines or mouse WT tissue.