STJ97394

antibody from St John's Laboratory

Targeting: CASP8 Casp-8, FLICE, MACH, MCH5

Immunocytochemistry

Antibody data

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Validation data

Product number: STJ97394 - Provider product page
Provider: St John's Laboratory
Product name: Anti-CASP8 antibody [2G12] (STJ97394)
Antibody type: Monoclonal
Description: Mouse monoclonal antibody anti-Caspase-8 is suitable for use in Western Blot, Immunofluorescence, Immunocytochemistry and Immunohistochemistry research applications.
Reactivity: Human, Mouse, Rat
Host: Mouse
Conjugate: Unconjugated
Antigen sequence: NA
Epitope: NA
Isotype: IgG
Antibody clone number: NA
Vial size: NA
Concentration: NA
Storage: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Handling: NA

CASP8 protein structure - STJ97394 shown in red.

Supportive validation

Submitted by: St John's Laboratory (provider)
Main image
Experimental details: Immunofluorescence analysis of Mouse-liver tissue. 1, Caspase-8 monoclonal antibody (2G12) (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
Sample type: NA
Validation comment: NA
Primary Ab dilution: NA
Other comments: NA
Secondary Ab: NA
Secondary Ab dilution: NA
Protocol: NA
Appendix: NA

Supportive validation

Submitted by: St John's Laboratory (provider)
Main image
Experimental details: Immunofluorescence analysis of Hela cell. 1, ER Alpha Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). Caspase-8 monoclonal antibody (2G12) (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min).
Sample type: NA
Validation comment: NA
Primary Ab dilution: NA
Other comments: NA
Secondary Ab: NA
Secondary Ab dilution: NA
Protocol: NA
Appendix: NA

Supportive validation

Submitted by: St John's Laboratory (provider)
Main image
Experimental details: Immunohistochemical analysis of paraffin-embedded Mouse Spleen Tissue using Caspase-8 monoclonal antibody.
Sample type: NA
Validation comment: NA
Primary Ab dilution: NA
Other comments: NA
Secondary Ab: NA
Secondary Ab dilution: NA
Protocol: NA
Appendix: NA

Supportive validation

Submitted by: St John's Laboratory (provider)
Main image
Experimental details: Immunohistochemical analysis of paraffin-embedded Mouse-brain tissue. 1, Caspase-8 monoclonal antibody (2G12) was diluted at 1:200 (4Â°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98Â°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
Sample type: NA
Validation comment: NA
Primary Ab dilution: NA
Other comments: NA
Secondary Ab: NA
Secondary Ab dilution: NA
Protocol: NA
Appendix: NA

Supportive validation

Submitted by: St John's Laboratory (provider)
Main image
Experimental details: Immunohistochemical analysis of paraffin-embedded Human-Tonsil tissue. 1, Caspase-8 monoclonal antibody (2G12) was diluted at 1:200 (4Â°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98Â°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
Sample type: NA
Validation comment: NA
Primary Ab dilution: NA
Other comments: NA
Secondary Ab: NA
Secondary Ab dilution: NA
Protocol: NA
Appendix: NA