PAB20049

antibody from Abnova Corporation

Targeting: CASP8 Casp-8, FLICE, MACH, MCH5

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB20049

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#PAB20049, RRID:AB_10964689

Product name

CASP8 polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against recombinant CASP8.

Antigen sequence

GIIYGTDGQEAPIYELTSQFTGLKCPSLAGKPKVF
FIQACQGDNYQKGIPVETDSEEQPYLEMDLSSPQT
RYIPDEADFLLGMATVNNCVSYRNPAEGTWYIQSL
CQSLRERCPRGDDILTILTEVNYEVSNKDDKKNMG
KQMPQP


Isotype

IgG

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of Lane 1: RT-4, Lane 2: U-251 MG, Lane 3: Human Plasma, Lane 4: Liver, Lane 5: Tonsil with CASP8 polyclonal antibody (Cat # PAB20049) at 1:250-1:500 dilution.

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line U-2 OS with CASP8 polyclonal antibody (Cat # PAB20049) at 1-4 ug/mL dilution shows positivity in nucleus but not nucleoli, cytoplasm.

Validation comment

Immunofluorescence

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunohistochemical staining of human kidney with CASP8 polyclonal antibody (Cat # PAB20049) shows distinct membranous and moderate cytoplasmic positivity in cells in tubules at 1:500-1:1000 dilution.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)