PAB20312

antibody from Abnova Corporation

Targeting: CASP8 Casp-8, FLICE, MACH, MCH5

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB20312

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#PAB20312, RRID:AB_10967287

Product name

CASP8 polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against recombinant CASP8.

Antigen sequence

LGEGKLDILKRVCAQINKSLLKIINDYEEFSKGEE
LCGVMTISDSPREQDSESQTLDKVYQMKSKPRGYC
LIINNHNFAKAREKVPKLHSIRDRNGTHLDAGALT
TTFEELHFEIKPHDDCTVEQIYEILKIYQLMDHSN
MDCFICC


Isotype

IgG

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of Lane 1: RT-4, Lane 2: U-251 MG, Lane 3: Human Plasma, Lane 4: Liver, Lane 5: Tonsil with CASP8 polyclonal antibody (Cat # PAB20312) at 1:250-1:500 dilution.

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line U-2 OS with CASP8 polyclonal antibody (Cat # PAB20312) at 1-4 ug/mL dilution shows positivity in mitochondria.

Validation comment

Immunofluorescence

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunohistochemical staining of human colon with CASP8 polyclonal antibody (Cat # PAB20312) shows strong cytoplasmic positivity in glandular cells at 1:50-1:200 dilution.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)