M00042-3

antibody from Boster Biological Technology

Targeting: CASP8 Casp-8, FLICE, MACH, MCH5

Western blot (Data presented on provider website)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

M00042-3

Provider

Boster Biological Technology

Product name

Anti-Caspase-8 Monoclonal Antibody (5F7)

Antibody type

Monoclonal

Description

Mouse Monoclonal 5F7 antibody for CASP8 detection. Tested positive for IHC, IP, WB in Human

Reactivity

Human

Host

Mouse

Antibody clone number

5F7

Vial size

100µg

Concentration

0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Storage

-20°C

Immunohistochemistry

Supportive validation

Submitted by

Boster Biological Technology (provider)

Main image

Experimental details

IHC analysis of CASP8 using anti-CASP8 antibody (M00042-3).CASP8 was detected in paraffin-embedded section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-CASP8 Antibody (M00042-3) overnight at 4°C. Biotinylated goat anti Mouse IgG antibody was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.

Additional image