622002

antibody from BioLegend
Targeting: CASP8 Casp-8, FLICE, MACH, MCH5
Provider product page for 622002
Western blot

Antibody data

Product number
622002 - Provider product page
Provider
BioLegend
Product name
Purified anti-Caspase-8
Antibody type
Monoclonal
Antigen
Recombinant full length human protein
Description
Caspase 8 (also known as ICE-like apoptotic protease 5, MORT1-associated CED-3 homolog (MACH), FADD-like ICE (FLICE), and apoptotic protease Mch-5) is a member of the peptidase family C14 that contains a death effector domain. Nine isoforms of this caspase have been reported, pro-Caspase 8 has a molecular weight of 55 kD. This caspase is found in the cytoplasm and, upon activation, in the mitochondria. Caspase 8 is the most upstream protease of caspase activation cascade, responsible for TNFRSF6/FAS/TRAIL-R mediated and TNFRSF1A induced cell death. This caspase cleaves/activates Caspase 3, Caspase 4, Caspase 6, Caspase 7, Caspase 9, Caspase 10, and Bid. Caspase 8 can be inhibited by the dominant negative isoform, ARC, and c-FLIP. Caspase 8 autocleaves into large p18 and small p10 subunits when activated by recruitment to the DISC complex at plasma membrane by FADD. This caspase interacts with FADD, CFLAR, and PEA15 proteins. Caspase 8 isoform 9 interacts at the ER with a complex containing BCAP31, BAP29, Bcl-2 and/or Bcl-2L1. Substrates for caspase 8 include androgen receptor, β-catenin, gelsolin, and vimentin. The 4-1-20 monoclonal antibody has been shown to be useful for Western blotting of human caspase 8 (55 kD pro-caspase form).
Reactivity
Human
Host
Mouse
Conjugate
Unconjugated
Isotype
IgG
Vial size
100 µg
Concentration
0.5 mg/ml
Storage
Upon receipt, store at 4° C.
CASP8 protein structure - 622002 shown in red.