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- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- 645501 - Provider product page
- Provider
- BioLegend
- Proper citation
- BioLegend Cat#645501, RRID:AB_2068466
- Product name
- Purified anti-Caspase-8
- Antibody type
- Monoclonal
- Antigen
- Full length caspase-8 fusion protein
- Description
- Caspase-8 is involved in the apoptosis mediated by CD95 and TNFR-1. It is suggested that following the triggering of apoptosis by CD95 or TNFR-1, the adaptor molecule FADD interact physically with cytosolic caspase-8 and initiate the apoptotic cascade. The active caspase-8 consists of two subunits of p18 and p10. This antibody should recognize the whole length of human caspase-8 and some isofrms of lower MW.
- Reactivity
- Human
- Host
- Rat
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 25 µg
- Concentration
- 0.5 mg/ml
- Storage
- Upon receipt, store at 4° C.
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Supportive validation
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- BioLegend (provider)
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- Experimental details
- Jurkat cell extract was resolved by electrophoresis, transferred to nitrocellulose, and probed with monoclonal anti-Caspase-8 (clone 1H10). Proteins were visualized using a goat anti-rat secondary conjugated to HRP and a chemiluminescent detection system.
- Conjugate
- Unconjugated
