MA5-11558

antibody from Invitrogen Antibodies

Targeting: CASP8 Casp-8, FLICE, MACH, MCH5

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Recommended by provider)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-11558

Provider

Invitrogen Antibodies

Product name

Caspase 8 Monoclonal Antibody (8CSP03 (4.1.20))

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Description

MA5-11558 targets Caspase 8 in IP and WB applications and shows reactivity with Human samples. The MA5-11558 immunogen is recombinant full length human Caspase 8 protein.

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

8CSP03 (4.1.20)

Vial size

500 µL

Concentration

0.2 mg/mL

Storage

4° C

References

Submitted references

Lactobacillus casei Strain Shirota Enhances the In Vitro Antiproliferative Effect of Geniposide in Human Oral Squamous Carcinoma HSC-3 Cells.

Qian Y, Song JL, Sun P, Yi R, Liu H, Feng X, Park KY, Zhao X
Molecules (Basel, Switzerland) 2018 May 3;23(5)

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Autocrine production of interleukin-4 and interleukin-10 is required for survival and growth of thyroid cancer cells.

Todaro M, Zerilli M, Ricci-Vitiani L, Bini M, Perez Alea M, Maria Florena A, Miceli L, Condorelli G, Bonventre S, Di Gesù G, De Maria R, Stassi G
Cancer research 2006 Feb 1;66(3):1491-9

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot of Caspase 8 using Caspase 8 Monoclonal Antibody (Product # MA5-11558) on Jurkat Cells.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis was performed on whole cell extracts (30 µg lysate) of Jurkat (Lane 1), Jurkat treated for overnight with 3 uM of Staurosporine (Lane 2), HeLa (Lane 3), HeLa treated for overnight with 3 uM of Staurosporine (lane 4), A549 (lane 5), PC-3 (lane 6) and MDA-MB-231 (lane 7). The blots were probed with Anti-Caspase 8 Mouse Monoclonal Antibody (Product # MA5-11558, 2-4 µg/mL) and detected by chemiluminescence using Goat anti-Mouse IgG (H+L) Secondary Antibody, HRP conjugate (Product # 62-6520, 1:4000 dilution). A 55 kDa band corresponding to Caspase 8 was observed across cell lines tested except Staurosporine treated lysates. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 10 % Bis-Tris gel (Product # NP0302BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® 2 Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary Antibody using iBind™ Flex Western Starter Kit (Product # SLF2000S). Chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate (Product # 32106).

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry analysis of Caspase 8 was done on MDA-MB-231 cells. Cells were fixed with 70% ethanol for 10 minutes, permeabilized with 0.25% Triton™ X-100 for 20 minutes, and blocked with 5% BSA for 30 minutes at room temperature. Cells were labeled with Caspase 8 Mouse Monoclonal Antibody (MA511558, red histogram) or with mouse isotype control (yellow histogram) at 3-5 ug/million cells in 2.5% BSA. After incubation at room temperature for 2 hours, the cells were labeled with Alexa Fluor® 488 Rabbit Anti-Mouse Secondary Antibody (A11059) at a dilution of 1:400 for 30 minutes at room temperature. The representative 10,000 cells were acquired and analyzed for each sample using an Attune® Acoustic Focusing Cytometer. The purple histogram represents unstained control cells and the green histogram represents no-primary-antibody control.