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ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [22]
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- Immunocytochemistry [1]
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- Product number
- ALX-804-242-C100 - Provider product page
- Provider
- Enzo Life Sciences
- Proper citation
- Enzo Life Sciences Cat#ALX-804-242-C100, RRID:AB_2050949
- Product name
- Caspase-8 (human) monoclonal antibody (12F5)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full length protein
- Description
- Protein G-affinity purified.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 12F5
- Vial size
- 100 μg
- Storage
- +4°C
- Handling
- Avoid freeze/thaw cycles.
Submitted references Cellular IAP proteins and LUBAC differentially regulate necrosome-associated RIP1 ubiquitination.
Intestinal genetic inactivation of caspase-8 diminishes migration of enterocytes.
Up-regulation of Bcl-2 during adipogenesis mediates apoptosis resistance in human adipocytes.
Prognostic and therapeutic relevance of FLIP and procaspase-8 overexpression in non-small cell lung cancer.
Vorinostat/SAHA-induced apoptosis in malignant mesothelioma is FLIP/caspase 8-dependent and HR23B-independent.
Cathepsin D primes caspase-8 activation by multiple intra-chain proteolysis.
A novel TNFR1-triggered apoptosis pathway mediated by class IA PI3Ks in neutrophils.
GDP-mannose-4,6-dehydratase (GMDS) deficiency renders colon cancer cells resistant to tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) receptor- and CD95-mediated apoptosis by inhibiting complex II formation.
Endogenous Bak inhibitors Mcl-1 and Bcl-xL: differential impact on TRAIL resistance in Bax-deficient carcinoma.
The antiviral adaptor proteins Cardif and Trif are processed and inactivated by caspases.
A protective role for the human SMG-1 kinase against tumor necrosis factor-alpha-induced apoptosis.
The prevention of spontaneous apoptosis of follicular lymphoma B cells by a follicular dendritic cell line: involvement of caspase-3, caspase-8 and c-FLIP.
Constitutive caspase activation and impaired death-inducing signaling complex formation in CD95-resistant, long-term activated, antigen-specific T cells.
An inducible pathway for degradation of FLIP protein sensitizes tumor cells to TRAIL-induced apoptosis.
Trail-induced apoptosis in Type I leukemic cells is not enhanced by overexpression of bax.
Fas-associated death domain protein (FADD) and caspase-8 mediate up-regulation of c-Fos by Fas ligand and tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) via a FLICE inhibitory protein (FLIP)-regulated pathway.
IL-10 induces apoptosis in human monocytes involving the CD95 receptor/ligand pathway.
Inhibition of death receptor-mediated gene induction by a cycloheximide-sensitive factor occurs at the level of or upstream of Fas-associated death domain protein (FADD).
Anticancer drugs induce caspase-8/FLICE activation and apoptosis in the absence of CD95 receptor/ligand interaction.
P2Z purinoreceptor ligation induces activation of caspases with distinct roles in apoptotic and necrotic alterations of cell death.
Sendai virus infection induces apoptosis through activation of caspase-8 (FLICE) and caspase-3 (CPP32).
Differential regulation and ATP requirement for caspase-8 and caspase-3 activation during CD95- and anticancer drug-induced apoptosis.
de Almagro MC, Goncharov T, Newton K, Vucic D
Cell death & disease 2015 Jun 25;6:e1800
Cell death & disease 2015 Jun 25;6:e1800
Intestinal genetic inactivation of caspase-8 diminishes migration of enterocytes.
Kaemmerer E, Kuhn P, Schneider U, Jeon MK, Klaus C, Schiffer M, Weisner D, Liedtke C, Jäkel J, Kennes LN, Hilgers RD, Wagner N, Gassler N
World journal of gastroenterology 2015 Apr 21;21(15):4499-508
World journal of gastroenterology 2015 Apr 21;21(15):4499-508
Up-regulation of Bcl-2 during adipogenesis mediates apoptosis resistance in human adipocytes.
Nagel SA, Keuper M, Zagotta I, Enlund E, Ruperez AI, Debatin KM, Wabitsch M, Fischer-Posovszky P
Molecular and cellular endocrinology 2014 Jan 25;382(1):368-76
Molecular and cellular endocrinology 2014 Jan 25;382(1):368-76
Prognostic and therapeutic relevance of FLIP and procaspase-8 overexpression in non-small cell lung cancer.
Riley JS, Hutchinson R, McArt DG, Crawford N, Holohan C, Paul I, Van Schaeybroeck S, Salto-Tellez M, Johnston PG, Fennell DA, Gately K, O'Byrne K, Cummins R, Kay E, Hamilton P, Stasik I, Longley DB
Cell death & disease 2013 Dec 5;4:e951
Cell death & disease 2013 Dec 5;4:e951
Vorinostat/SAHA-induced apoptosis in malignant mesothelioma is FLIP/caspase 8-dependent and HR23B-independent.
Hurwitz JL, Stasik I, Kerr EM, Holohan C, Redmond KM, McLaughlin KM, Busacca S, Barbone D, Broaddus VC, Gray SG, O'Byrne KJ, Johnston PG, Fennell DA, Longley DB
European journal of cancer (Oxford, England : 1990) 2012 May;48(7):1096-107
European journal of cancer (Oxford, England : 1990) 2012 May;48(7):1096-107
Cathepsin D primes caspase-8 activation by multiple intra-chain proteolysis.
Conus S, Pop C, Snipas SJ, Salvesen GS, Simon HU
The Journal of biological chemistry 2012 Jun 15;287(25):21142-51
The Journal of biological chemistry 2012 Jun 15;287(25):21142-51
A novel TNFR1-triggered apoptosis pathway mediated by class IA PI3Ks in neutrophils.
Geering B, Gurzeler U, Federzoni E, Kaufmann T, Simon HU
Blood 2011 Jun 2;117(22):5953-62
Blood 2011 Jun 2;117(22):5953-62
GDP-mannose-4,6-dehydratase (GMDS) deficiency renders colon cancer cells resistant to tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) receptor- and CD95-mediated apoptosis by inhibiting complex II formation.
Moriwaki K, Shinzaki S, Miyoshi E
The Journal of biological chemistry 2011 Dec 16;286(50):43123-33
The Journal of biological chemistry 2011 Dec 16;286(50):43123-33
Endogenous Bak inhibitors Mcl-1 and Bcl-xL: differential impact on TRAIL resistance in Bax-deficient carcinoma.
Gillissen B, Wendt J, Richter A, Richter A, Müer A, Overkamp T, Gebhardt N, Preissner R, Belka C, Dörken B, Daniel PT
The Journal of cell biology 2010 Mar 22;188(6):851-62
The Journal of cell biology 2010 Mar 22;188(6):851-62
The antiviral adaptor proteins Cardif and Trif are processed and inactivated by caspases.
Rebsamen M, Meylan E, Curran J, Tschopp J
Cell death and differentiation 2008 Nov;15(11):1804-11
Cell death and differentiation 2008 Nov;15(11):1804-11
A protective role for the human SMG-1 kinase against tumor necrosis factor-alpha-induced apoptosis.
Oliveira V, Romanow WJ, Geisen C, Otterness DM, Mercurio F, Wang HG, Dalton WS, Abraham RT
The Journal of biological chemistry 2008 May 9;283(19):13174-84
The Journal of biological chemistry 2008 May 9;283(19):13174-84
The prevention of spontaneous apoptosis of follicular lymphoma B cells by a follicular dendritic cell line: involvement of caspase-3, caspase-8 and c-FLIP.
Goval JJ, Thielen C, Bourguignon C, Greimers R, Dejardin E, Choi YS, Boniver J, de Leval L
Haematologica 2008 Aug;93(8):1169-77
Haematologica 2008 Aug;93(8):1169-77
Constitutive caspase activation and impaired death-inducing signaling complex formation in CD95-resistant, long-term activated, antigen-specific T cells.
Strauss G, Knape I, Melzner I, Debatin KM
Journal of immunology (Baltimore, Md. : 1950) 2003 Aug 1;171(3):1172-82
Journal of immunology (Baltimore, Md. : 1950) 2003 Aug 1;171(3):1172-82
An inducible pathway for degradation of FLIP protein sensitizes tumor cells to TRAIL-induced apoptosis.
Kim Y, Suh N, Sporn M, Reed JC
The Journal of biological chemistry 2002 Jun 21;277(25):22320-9
The Journal of biological chemistry 2002 Jun 21;277(25):22320-9
Trail-induced apoptosis in Type I leukemic cells is not enhanced by overexpression of bax.
Jia L, Patwari Y, Kelsey SM, Newland AC
Biochemical and biophysical research communications 2001 May 25;283(5):1037-45
Biochemical and biophysical research communications 2001 May 25;283(5):1037-45
Fas-associated death domain protein (FADD) and caspase-8 mediate up-regulation of c-Fos by Fas ligand and tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) via a FLICE inhibitory protein (FLIP)-regulated pathway.
Siegmund D, Mauri D, Peters N, Juo P, Thome M, Reichwein M, Blenis J, Scheurich P, Tschopp J, Wajant H
The Journal of biological chemistry 2001 Aug 31;276(35):32585-90
The Journal of biological chemistry 2001 Aug 31;276(35):32585-90
IL-10 induces apoptosis in human monocytes involving the CD95 receptor/ligand pathway.
Schmidt M, Lügering N, Pauels HG, Schulze-Osthoff K, Domschke W, Kucharzik T
European journal of immunology 2000 Jun;30(6):1769-77
European journal of immunology 2000 Jun;30(6):1769-77
Inhibition of death receptor-mediated gene induction by a cycloheximide-sensitive factor occurs at the level of or upstream of Fas-associated death domain protein (FADD).
Wajant H, Haas E, Schwenzer R, Muhlenbeck F, Kreuz S, Schubert G, Grell M, Smith C, Scheurich P
The Journal of biological chemistry 2000 Aug 11;275(32):24357-66
The Journal of biological chemistry 2000 Aug 11;275(32):24357-66
Anticancer drugs induce caspase-8/FLICE activation and apoptosis in the absence of CD95 receptor/ligand interaction.
Wesselborg S, Engels IH, Rossmann E, Los M, Schulze-Osthoff K
Blood 1999 May 1;93(9):3053-63
Blood 1999 May 1;93(9):3053-63
P2Z purinoreceptor ligation induces activation of caspases with distinct roles in apoptotic and necrotic alterations of cell death.
Ferrari D, Los M, Bauer MK, Vandenabeele P, Wesselborg S, Schulze-Osthoff K
FEBS letters 1999 Mar 19;447(1):71-5
FEBS letters 1999 Mar 19;447(1):71-5
Sendai virus infection induces apoptosis through activation of caspase-8 (FLICE) and caspase-3 (CPP32).
Bitzer M, Prinz F, Bauer M, Spiegel M, Neubert WJ, Gregor M, Schulze-Osthoff K, Lauer U
Journal of virology 1999 Jan;73(1):702-8
Journal of virology 1999 Jan;73(1):702-8
Differential regulation and ATP requirement for caspase-8 and caspase-3 activation during CD95- and anticancer drug-induced apoptosis.
Ferrari D, Stepczynska A, Los M, Wesselborg S, Schulze-Osthoff K
The Journal of experimental medicine 1998 Sep 7;188(5):979-84
The Journal of experimental medicine 1998 Sep 7;188(5):979-84
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- Enzo Life Sciences (provider)
- Main image
- Experimental details
- . (A) Schematic structure of caspase-8 and its cleavage products as detected by anti-caspase-8 (12F5). The antibody is directed against the p18 subunit and detects procaspase-8 (p55/54), the intermediate cleavage products of 43kD and 41kD and the p18 active subunit of caspase-8. (B) Detection of Fas ligand-induced caspase-8 processing and activation in human Jurkat cells. Method: Jurkat cells were treated with Fas ligand (100ng/ml). After the indicated time points cell lysates were prepared and separated on a 12.5% SDS-PAGE (2x10E6 cells/lane) under reducing conditions. Proteins were immunoblotted with anti-caspase-8 12F5 (1µg/ml). Following incubation with peroxidase-conjugated secondary antibodies caspase-8 processing was detected by enhanced chemoluminescent staining. The open arrowhead indicates the two isoforms of procaspase-8 (caspase-8/a and caspase-8/b), which are cleaved into the intermediate forms p43 and p41 and finally processed to the active p18 subunit (filled arrowheads).
