ALX-804-448-C100

antibody from Enzo Life Sciences

Targeting: CASP8 Casp-8, FLICE, MACH, MCH5

Western blot (Data presented on provider website)

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Flow cytometry (Data presented on provider website)

Antibody Data

Product number

ALX-804-448-C100

Provider

Enzo Life Sciences

Proper citation

Enzo Life Sciences Cat#ALX-804-448-C100, RRID:AB_2050953

Product name

Caspase-8 (mouse) monoclonal antibody (3B10)

Antibody type

Monoclonal

Antigen

Recombinant full length protein

Description

Protein G-affinity purified.

Reactivity

Mouse

Host

Rat

Isotype

IgG

Antibody clone number

3B10

Vial size

100 μg

Storage

-20°C

Handling

For long term storage, aliquot and freeze at -20¡C. Avoid freeze/thaw cycles.

References

Submitted references

Caspase-8 deficiency in epidermal keratinocytes triggers an inflammatory skin disease.

Kovalenko A, Kim JC, Kang TB, Rajput A, Bogdanov K, Dittrich-Breiholz O, Kracht M, Brenner O, Wallach D
The Journal of experimental medicine 2009 Sep 28;206(10):2161-77

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Membrane-bound Fas ligand only is essential for Fas-induced apoptosis.

O' Reilly LA, Tai L, Lee L, Kruse EA, Grabow S, Fairlie WD, Haynes NM, Tarlinton DM, Zhang JG, Belz GT, Smyth MJ, Bouillet P, Robb L, Strasser A
Nature 2009 Oct 1;461(7264):659-63

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Impaired lactation in mice expressing dominant-negative FADD in mammary epithelium.

Shackleton M, O'Reilly LA, Sutherland KD, Bath ML, Ellis S, Strasser A, Visvader JE, Lindeman GJ
Developmental dynamics : an official publication of the American Association of Anatomists 2009 Apr;238(4):1010-6

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Stimulation of Toll-like receptor 3 and 4 induces interleukin-1beta maturation by caspase-8.

Maelfait J, Vercammen E, Janssens S, Schotte P, Haegman M, Magez S, Beyaert R
The Journal of experimental medicine 2008 Sep 1;205(9):1967-73

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Modifications and intracellular trafficking of FADD/MORT1 and caspase-8 after stimulation of T lymphocytes.

O'Reilly LA, Divisekera U, Newton K, Scalzo K, Kataoka T, Puthalakath H, Ito M, Huang DC, Strasser A
Cell death and differentiation 2004 Jul;11(7):724-36

Immunocytochemistry

Supportive validation

Submitted by

Enzo Life Sciences (provider)

Main image

Experimental details

Flow cytometry of HEK 293T cells transfected with FLAG tagged mutated mouse caspase-8. Method: MAbs 1G12 and 3B10 both detect overexpressed FLAG-epitope-tagged pro-caspase-8 (active-site-mutant) by flow cytometry in permeabilized cells. As a positive control staining with a MAb to FLAG was performed. As a negative control an isotype control was employed.

Supportive validation

Submitted by

Enzo Life Sciences (provider)

Main image

Experimental details

MAbs 1G12 and 3B10 both detect pro-caspase-8 in MEFs from WT mice, but not in MEFs from caspase-8-/- mice. Several smaller bands detected in the caspase-8-/- MEFs, correspond to truncated forms of caspase-8 made in the caspase-8-/- mice since only exons 1 and 2 of mouse caspase-8 were deleted in these knock-out mice and not the region encoding the p18 subunit.Note: Extra bands marked by * are only seen in lysates from caspase-8-/- MEFs and not in lysates from any WT cell lines or mouse WT tissue. Both MAbs to caspase-8 (mouse) do not recognize human caspase-8, whereas endogenous caspase-8 can be efficiently detected in various mouse cell lines. Upon an apoptotic stimulus e.g. by cross-linked rhsFasL both MAbs to caspase-8 (mouse) do also recognize the cleaved active p18 subunit of mouse caspase-8 in addition to the caspase-8 precursor.