LS-C180848

antibody from LSBio

Targeting: BTK AGMX1, ATK, IMD1, PSCTK1, XLA

Western blot (Enhanced validation)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Enhanced validation)

Antibody Data

Product number

LS-C180848

Provider

LSBio

Product name

BTK Antibody (phospho-Tyr223, DY488) LS-C180848

Antibody type

Polyclonal

Description

Affinity purified

Reactivity

Human, Mouse

Host

Rabbit

Conjugate

Green dye

Storage

Store at 4°C in the dark.

Western blot

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Western Blot: BTK [p Tyr223] Antibody - WB analysis of BTKpY223 with peptide competition: 1. ab only, 2. 200 molar excess unmodified immunizing peptide and 3. 200 molar excess modified immunizing peptide. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunocytochemistry

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Immunocytochemistry/Immunofluorescence: BTK [p Tyr223] Antibody - Antibody was tested at 1:50 in Jurkat cells with FITC (green). Nuclei were counterstained with DAPI (blue). This image was taken for the unconjugated form of this product. Other forms have not been tested.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Immunocytochemistry/Immunofluorescence: BTK [p Tyr223] Antibody - Antibody was tested at 1:50 in Jurkat cells with FITC (green). Nuclei were counterstained with DAPI (blue). This image was taken for the unconjugated form of this product. Other forms have not been tested.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Immunocytochemistry/Immunofluorescence: BTK [p Tyr223] Antibody - Antibody was tested at 1:50 in Jurkat cells with FITC (green). Nuclei were counterstained with DAPI (blue). This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Immunohistochemistry: BTK [p Tyr223] Antibody - IHC of BTK in kidney cancer xenograft using DAB with hematoxylin counterstain. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Immunohistochemistry: BTK [p Tyr223] Antibody - IHC of BTK in kidney cancer xenograft using DAB with hematoxylin counterstain. This image was taken for the unconjugated form of this product. Other forms have not been tested.