GTX61791

antibody from GeneTex

Targeting: BTK AGMX1, ATK, IMD1, PSCTK1, XLA

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Antibody Data

Product number

GTX61791

Provider

GeneTex

Proper citation

GeneTex Cat#GTX61791, RRID:AB_10622447

Product name

Btk (phospho Tyr223) antibody [EP420Y]

Antibody type

Monoclonal

Reactivity

Human, Mouse

Host

Rabbit

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

References

Submitted references

CTRP3 acts as a negative regulator of osteoclastogenesis through AMPK-c-Fos-NFATc1 signaling in vitro and RANKL-induced calvarial bone destruction in vivo.

Kim JY, Min JY, Baek JM, Ahn SJ, Jun HY, Yoon KH, Choi MK, Lee MS, Oh J
Bone 2015 Oct;79:242-51

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Aconitum pseudo-laeve var. erectum inhibits receptor activator of nuclear factor kappa-B ligand-induced osteoclastogenesis via the c-Fos/nuclear factor of activated T-cells, cytoplasmic 1 signaling pathway and prevents lipopolysaccharide-induced bone loss in mice.

Baek JM, Kim JY, Cheon YH, Park SH, Ahn SJ, Yoon KH, Oh J, Lee MS
Molecules (Basel, Switzerland) 2014 Aug 5;19(8):11628-44

Western blot

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

A. Western blot analysis on Ramos cell lysates using anti-Phospho-BTK (pY223) RabMAb (cat. #GTX61791) 1:500000 dilution. Cells were either (A) untreated (B) treated with pervanadate.

Validation comment

WB

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

WB analysis of lysates from (1) untreated and pervanadate-treated Ramos cells (10 μg per lane) using Btk (phospho Tyr223) antibody [EP420Y] at a dilution of 1:500,000.