Antibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PAB3419 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB3419, RRID:AB_1571800
- Product name
- BTK polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of BTK.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Conformation of full-length Bruton tyrosine kinase (Btk) from synchrotron X-ray solution scattering.
Bruton's tyrosine kinase is required for lipopolysaccharide-induced tumor necrosis factor alpha production.
Bruton's tyrosine kinase is a Toll/interleukin-1 receptor domain-binding protein that participates in nuclear factor kappaB activation by Toll-like receptor 4.
Márquez JA, Smith CI, Petoukhov MV, Lo Surdo P, Mattsson PT, Knekt M, Westlund A, Scheffzek K, Saraste M, Svergun DI
The EMBO journal 2003 Sep 15;22(18):4616-24
The EMBO journal 2003 Sep 15;22(18):4616-24
Bruton's tyrosine kinase is required for lipopolysaccharide-induced tumor necrosis factor alpha production.
Horwood NJ, Mahon T, McDaid JP, Campbell J, Mano H, Brennan FM, Webster D, Foxwell BM
The Journal of experimental medicine 2003 Jun 16;197(12):1603-11
The Journal of experimental medicine 2003 Jun 16;197(12):1603-11
Bruton's tyrosine kinase is a Toll/interleukin-1 receptor domain-binding protein that participates in nuclear factor kappaB activation by Toll-like receptor 4.
Jefferies CA, Doyle S, Brunner C, Dunne A, Brint E, Wietek C, Walch E, Wirth T, O'Neill LA
The Journal of biological chemistry 2003 Jul 11;278(28):26258-64
The Journal of biological chemistry 2003 Jul 11;278(28):26258-64
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- The BTK polyclonal antibody (Cat # PAB3419) is used in Western blot to detect BTK in Ramos cell lysate.
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of BTK polyclonal antibody (Cat # PAB3419) transiently transfected HEK293 cell line lysate (1 ug/lane). BTK polyclonal antibody (arrow) was detected using the purified polyclonal antibody (1 : 1500 dilution).
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human cancer tissue reacted with BTK polyclonal antibody (Cat # PAB3419) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. BC = breast carcinoma.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)