601701

antibody from BioLegend

Targeting: BTK AGMX1, ATK, IMD1, PSCTK1, XLA

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

601701

Provider

BioLegend

Proper citation

BioLegend Cat#601701, RRID:AB_2715906

Product name

Purified anti-BTK Phospho (Tyr 223) antibody

Antibody type

Monoclonal

Reactivity

Human

Host

Mouse

Conjugate

Unconjugated

Antibody clone number

A16128B

Vial size

25 µg

Concentration

0.5 mg/ml

Storage

2°C-8°C

Handling

Ambient RT

Western blot

Supportive validation

Submitted by

BioLegend (provider)

Main image

Experimental details

Total cell lysates (15 µg protein) from Ramos cells treated with (+) or without (-) 2 mM H₂O₂ for 3 minutes were resolved by 4-20% Tris-Glycine gel electrophoresis, transferred to nitrocellulose, and probed with 2 µg/mL (1:250) purified anti-BTK Phospho (Tyr 223) antibody (Clone A16128B, left) or a competitor’s clone used at the manufacturer’s recommended concentration (right). Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse-IgG (clone Poly4053) for clone A16128B, and HRP donkey anti-rabbit IgG (clone Poly4064) for the competitor’s clone, each at a 1:3000 dilution. Equal BTK loading was confirmed using a pan-BTK antibody.

Conjugate

Unconjugated