711720

antibody from Invitrogen Antibodies

Targeting: BTK AGMX1, ATK, IMD1, PSCTK1, XLA

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

711720

Provider

Invitrogen Antibodies

Product name

Phospho-Btk (Tyr223) Recombinant Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µg

Concentration

0.5 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

For immunofluorescence analysis, RAMOS cells were fixed and permeabilized for detection of endogenous BTK pY223 using Anti-BTK pY223 Recombinant Rabbit Polyclonal Antibody (Product # 711720, 2 µg/mL) and labeled with Goat anti-Rabbit IgG (H+L) Superclonal Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 1:2000). Panel a) shows representative cells that were stained for detection and localization of BTK pY223 protein (green), Panel b) is stained for nuclei (blue) using SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). Panel c) represents cytoskeletal F-actin staining using Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300). Panel d) is a composite image of Panels a, b and c clearly demonstrating cytoplasmic localization of BTK pY223. Antibody specificity was demonstrated by competition with the BTK pY223 phospho peptide, which results in loss of signal in panel e). Panel f) shows no competition with the non-phospho peptide. The images were captured at 60X magnification