Antibody data
- Antibody Data
- Antigen structure
- References [4]
- Comments [0]
- Validations
- Immunocytochemistry [1]
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- Product number
- 50-9015-80 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-Phospho-BTK/ITK (Tyr551, Tyr511) Monoclonal Antibody (M4G3LN), eFluor 660, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: This M4G3LN monoclonal antibody recognizes human and mouse Bruton's tyrosine kinase (also known as BTK, agammaglobulinaemia tyrosine kinase, B-cell progenitor kinase)/interleukin-2-inducible tyrosine kinase (also known as ITK, T-cell-specific kinase, Tyrosine-protein kinase Lyk) when phosphorylated on Y551 and Y511, respectively. In addition to Src family and SYK family kinases, B cells and T cells express a third family of receptor-proximal kinases called the Tec kinase family. The B cell-specific Tec family kinase is BTK; T cells express ITK as well as Tec and Rlk. Tec family kinases play an indispensable role in activation of phospholipase C and activation of calcium-dependent signaling cascades following antigen receptor triggering. BTK has been implicated in propagating signals downstream of LPS-TLR4 in B cells via signaling through the TLR4 co-receptor RP105/CD180. Mice congenitally lacking BTK or ITK show profound defects in B cell or T cell development and signaling, respectively. People with mutations or deletions in BTK suffer from X-linked agammaglobulinemia and X-linked immunodeficiency. Specificity of this M4G3LN clone was determined by ELISA, flow cytometry, and western blotting. Applications Reported: This M4G3LN antibody has been reported for use in immunohistochemical staining, and immunohistochemical staining of formalin-fixed paraffin embedded tissue sections. Applications Tested: This M4G3LN antibody has been tested by immunohistochemistry of formalin-fixed paraffin embedded human tissue using high pH antigen retrieval and can be used at less than or equal to10 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. eFluor® 660 is a replacement for Alexa Fluor® 647. eFluor® 660 emits at 659 nm and is excited with the red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome. Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser. Filtration: 0.2 µm post-manufacturing filtered.
- Reactivity
- Human, Mouse
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- M4G3LN
- Vial size
- 25 µg
- Concentration
- 0.2 mg/mL
- Storage
- 4° C, store in dark, DO NOT FREEZE!
Submitted references Activation loop dynamics determine the different catalytic efficiencies of B cell- and T cell-specific tec kinases.
TCR signaling via Tec kinase ITK and interferon regulatory factor 4 (IRF4) regulates CD8+ T-cell differentiation.
T-cell signaling regulated by the Tec family kinase, Itk.
Bruton's tyrosine kinase mediates NF-kappa B activation and B cell survival by B cell-activating factor receptor of the TNF-R family.
Joseph RE, Kleino I, Wales TE, Xie Q, Fulton DB, Engen JR, Berg LJ, Andreotti AH
Science signaling 2013 Aug 27;6(290):ra76
Science signaling 2013 Aug 27;6(290):ra76
TCR signaling via Tec kinase ITK and interferon regulatory factor 4 (IRF4) regulates CD8+ T-cell differentiation.
Nayar R, Enos M, Prince A, Shin H, Hemmers S, Jiang JK, Klein U, Thomas CJ, Berg LJ
Proceedings of the National Academy of Sciences of the United States of America 2012 Oct 9;109(41):E2794-802
Proceedings of the National Academy of Sciences of the United States of America 2012 Oct 9;109(41):E2794-802
T-cell signaling regulated by the Tec family kinase, Itk.
Andreotti AH, Schwartzberg PL, Joseph RE, Berg LJ
Cold Spring Harbor perspectives in biology 2010 Jul;2(7):a002287
Cold Spring Harbor perspectives in biology 2010 Jul;2(7):a002287
Bruton's tyrosine kinase mediates NF-kappa B activation and B cell survival by B cell-activating factor receptor of the TNF-R family.
Shinners NP, Carlesso G, Castro I, Hoek KL, Corn RA, Woodland RT, Scott ML, Wang D, Khan WN
Journal of immunology (Baltimore, Md. : 1950) 2007 Sep 15;179(6):3872-80
Journal of immunology (Baltimore, Md. : 1950) 2007 Sep 15;179(6):3872-80
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Supportive validation
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- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of formalin-fixed paraffin embedded human tonsil tissue using 10 µg/mL Mouse IgG2b K Isotype Control eFluor® 660 (left) or 10 µg/mL Anti-Human/Mouse phospho-BTK/ITK (Y551/Y511) eFluor® 660 (right). Nuclei are stained with DAPI.