50-9015-80

antibody from Invitrogen Antibodies
Targeting: BTK AGMX1, ATK, IMD1, PSCTK1, XLA
Provider product page for 50-9015-80
Immunocytochemistry
Immunohistochemistry

Antibody data

Product number
50-9015-80 - Provider product page
Provider
Invitrogen Antibodies
Product name
Anti-Phospho-BTK/ITK (Tyr551, Tyr511) Monoclonal Antibody (M4G3LN), eFluor 660, eBioscience™
Antibody type
Monoclonal
Antigen
Other
Description
Description: This M4G3LN monoclonal antibody recognizes human and mouse Bruton's tyrosine kinase (also known as BTK, agammaglobulinaemia tyrosine kinase, B-cell progenitor kinase)/interleukin-2-inducible tyrosine kinase (also known as ITK, T-cell-specific kinase, Tyrosine-protein kinase Lyk) when phosphorylated on Y551 and Y511, respectively. In addition to Src family and SYK family kinases, B cells and T cells express a third family of receptor-proximal kinases called the Tec kinase family. The B cell-specific Tec family kinase is BTK; T cells express ITK as well as Tec and Rlk. Tec family kinases play an indispensable role in activation of phospholipase C and activation of calcium-dependent signaling cascades following antigen receptor triggering. BTK has been implicated in propagating signals downstream of LPS-TLR4 in B cells via signaling through the TLR4 co-receptor RP105/CD180. Mice congenitally lacking BTK or ITK show profound defects in B cell or T cell development and signaling, respectively. People with mutations or deletions in BTK suffer from X-linked agammaglobulinemia and X-linked immunodeficiency. Specificity of this M4G3LN clone was determined by ELISA, flow cytometry, and western blotting. Applications Reported: This M4G3LN antibody has been reported for use in immunohistochemical staining, and immunohistochemical staining of formalin-fixed paraffin embedded tissue sections. Applications Tested: This M4G3LN antibody has been tested by immunohistochemistry of formalin-fixed paraffin embedded human tissue using high pH antigen retrieval and can be used at less than or equal to10 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. eFluor® 660 is a replacement for Alexa Fluor® 647. eFluor® 660 emits at 659 nm and is excited with the red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome. Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser. Filtration: 0.2 µm post-manufacturing filtered.
Reactivity
Human, Mouse
Host
Mouse
Isotype
IgG
Antibody clone number
M4G3LN
Vial size
25 µg
Concentration
0.2 mg/mL
Storage
4° C, store in dark, DO NOT FREEZE!
BTK protein structure - 50-9015-80 shown in red.