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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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Validation data
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- Product number
- TA501100 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#TA501100, RRID:AB_11125446
- Product name
- Anti-BTK mouse monoclonal antibody, clone OTI3E9 (formerly 3E9)
- Antibody type
- Monoclonal
- Description
- Anti-BTK mouse monoclonal antibody, clone OTI3E9 (formerly 3E9)
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- BTK
- Isotype
- IgG
- Antibody clone number
- OTI3E9
- Vial size
- 100 µl
- Concentration
- 1 mg/ml
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BTK (RC211582, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BTK.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Anti-BTK mouse monoclonal antibody (TA501100) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY BTK(RC211582).
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Kidney tissue within the normal limits using anti-BTK mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501100, Dilution 1:50)
- Validation comment
- IHC
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells transfected with either pCMV6-ENTRY BTK(RC211582)(Red) or empty vector control plasmid(Blue) were immunostained with anti-BTK mouse monoclonal(TA501100), and then analyzed by flow cytometry.
- Validation comment
- FC
