TA501102

antibody from OriGene

Targeting: BTK AGMX1, ATK, IMD1, PSCTK1, XLA

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

TA501102

Provider

OriGene

Proper citation

OriGene Cat#TA501102, RRID:AB_11125237

Product name

Anti-BTK mouse monoclonal antibody, clone OTI13E5 (formerly 13E5)

Antibody type

Monoclonal

Description

Anti-BTK mouse monoclonal antibody, clone OTI13E5 (formerly 13E5)

Host

Mouse

Conjugate

Unconjugated

Epitope

BTK

Isotype

IgG

Antibody clone number

OTI13E5

Vial size

100 µl

Concentration

0.95 mg/ml

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BTK (RC211582, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BTK.

Validation comment

WB

Immunocytochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Anti-BTK mouse monoclonal antibody (TA501102) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY BTK(RC211582).

Validation comment

IF

Flow cytometry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

HEK293T cells transfected with either pCMV6-ENTRY BTK(RC211582)(Red) or empty vector control plasmid(Blue) were immunostained with anti-BTK mouse monoclonal(TA501102), and then analyzed by flow cytometry.

Validation comment

FC