14-9015-37

antibody from Invitrogen Antibodies

Targeting: BTK AGMX1, ATK, IMD1, PSCTK1, XLA

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

14-9015-37

Provider

Invitrogen Antibodies

Product name

Phospho-BTK/ITK (Tyr551, Tyr511) Monoclonal Antibody (M4G3LN), eBioscience™

Antibody type

Monoclonal

Antigen

Other

Description

Description: This M4G3LN monoclonal antibody recognizes human and mouse Bruton's tyrosine kinase (also known as BTK, agammaglobulinaemia tyrosine kinase, B-cell progenitor kinase)/interleukin-2-inducible tyrosine kinase (also known as ITK, T-cell-specific kinase, Tyrosine-protein kinase Lyk) when phosphorylated on Y551 and Y511, respectively.

Antibody clone number

M4G3LN

Concentration

0.5 mg/mL

References

Submitted references

Contribution of plasma cells and B cells to hidradenitis suppurativa pathogenesis.

Gudjonsson JE, Tsoi LC, Ma F, Billi AC, van Straalen KR, Vossen ARJV, van der Zee HH, Harms PW, Wasikowski R, Yee CM, Rizvi SM, Xing X, Xing E, Plazyo O, Zeng C, Patrick MT, Lowe MM, Burney RE, Kozlow JH, Cherry-Bukowiec JR, Jiang Y, Kirma J, Weidinger S, Cushing KC, Rosenblum MD, Berthier C, MacLeod AS, Voorhees JJ, Wen F, Kahlenberg JM, Maverakis E, Modlin RL, Prens EP
JCI insight 2020 Oct 2;5(19)

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunoblotting of reduced lysates from normal human peripheral blood cells treated with hydrogen peroxide-activated sodium orthovanadate (lane 1) or left untreated (lane 2), and probed with 5 µg/mL of Anti-Human/Mouse Phospho-BTK/ITK (Y551/Y511) Purified.Bands were visualized using Rat Anti-Mouse IgG HRP.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of formalin-fixed paraffin embedded human tonsil using 0.5 µg/mL of Anti-Human/Mouse Phospho-BTK/ITK (Y551/Y511) Purified, followed by Anti-Mouse IgG Biotin, Streptavidin HRP and DAB visualization.Nuclei are counterstained with hematoxylin.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 8 Enrichment and activation of B cell-associated signaling pathways in HS skin. Analysis of the signal transduction networks using literature-based networks (Genomatix-Pathway System, GePS) demonstrated enrichment for pathways involved in B cell signaling and activation ( A ). To confirm the nature of the inflammatory infiltrate in HS and the localization of components of the enriched signaling pathways, we performed IHC in an excisional biopsy for CD3, CD20, and CD138. Plasma cells were the predominant inflammatory infiltrate and most prominent in the deeper layers of the skin surrounding a deeper sinus tract ( A ), accompanied by increased expression of BTK, SYK, and LCK ( B ) ( n = 3). Activation of key components of this signaling pathway was confirmed by IHC for both phospho-BTK and phospho-SYK ( n = 3) ( C ).