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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
- Immunohistochemistry [7]
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- Product number
- NBP1-89206 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-89206, RRID:AB_11021862
- Product name
- Rabbit Polyclonal BTK Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Specificity of human BTK antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: BTK Antibody [NBP1-89206] - Lane 1: Marker [kDa] 219, 112, 85, 49, 32, 25, 18. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human cell line A-431. Lane 5: Human liver tissue. Lane 6: Human tonsil tissue
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: BTK Antibody [NBP1-89206] - Analysis using Anti-BTK antibody NBP1-89206 (A) shows similar pattern to independent antibody NBP1-89207 (B).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: BTK Antibody [NBP1-89206] - Analysis using Anti-BTK antibody NBP1-89206 (A) shows similar pattern to independent antibody NBP1-89207 (B).
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: BTK Antibody [NBP1-89206] - Staining of human kidney shows low expression as expected.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: BTK Antibody [NBP1-89206] - Staining of human lymph node shows high expression.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: BTK Antibody [NBP1-89206] - Staining of human liver shows no positivity in hepatocytes as expected.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: BTK Antibody [NBP1-89206] - Staining of human rectum shows moderate membranous positivity in lymphoid cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: BTK Antibody [NBP1-89206] - Staining of human skeletal muscle shows no positivity in myocytes as expected.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: BTK Antibody [NBP1-89206] - Staining in human tonsil and skeletal muscle tissues using NBP1-89206 antibody. Corresponding BTK RNA-seq data are presented for the same tissues.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: BTK Antibody [NBP1-89206] - Staining of human tonsil shows strong membranous positivity in non-germinal center cells.
